Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2039 linC

2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (2,5-DDOL dehydrogenase) (NCBI)

CircVis
Functional Annotations (9)
Function System
Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) cog/ cog
metabolic process go/ biological_process
oxidoreductase activity go/ molecular_function
Fructose and mannose metabolism kegg/ kegg pathway
Bisphenol degradation kegg/ kegg pathway
Chloroalkane and chloroalkene degradation kegg/ kegg pathway
Butanoate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2039
(Mouseover regulator name to see its description)

RSP_2039 is regulated by 15 influences and regulates 0 modules.
Regulators for RSP_2039 linC (15)
Regulator Module Operator
RSP_2027 11 tf
RSP_2922 11 tf
RSP_3001 11 tf
RSP_3385 11 tf
RSP_3418 11 tf
RSP_0457 120 tf
RSP_1274 120 tf
RSP_1892 120 tf
RSP_1925 120 tf
RSP_2681 120 tf
RSP_2853 120 tf
RSP_2922 120 tf
RSP_3094 120 tf
RSP_3667 120 tf
RSP_3686 120 tf

Warning: RSP_2039 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7742 7.80e-02 t.aaacca.TT.Attt
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7743 7.00e+00 TcGGgAcctccTG.cg.AaG
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7960 2.10e+02 Ga.cAGCCGGA.GAA
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7961 1.50e+03 CGtcccaGagGcaAC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2039

RSP_2039 is enriched for 9 functions in 3 categories.
Enrichment Table (9)
Function System
Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) cog/ cog
metabolic process go/ biological_process
oxidoreductase activity go/ molecular_function
Fructose and mannose metabolism kegg/ kegg pathway
Bisphenol degradation kegg/ kegg pathway
Chloroalkane and chloroalkene degradation kegg/ kegg pathway
Butanoate metabolism kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
Microbial metabolism in diverse environments kegg/ kegg pathway
Module neighborhood information for RSP_2039

RSP_2039 has total of 21 gene neighbors in modules 11, 120
Gene neighbors (21)
Gene Common Name Description Module membership
RSP_0337 RSP_0337 possible phospodiesterase (NCBI) 120, 215
RSP_0338 RSP_0338 hypothetical protein (NCBI) 120, 215
RSP_1196 RSP_1196 SAM-dependent methyltransferase (NCBI) 120, 152
RSP_1400 RSP_1400 DNA polymerase IV (RefSeq) 11, 133
RSP_1916 RSP_1916 possible 2-hydroxychromene-2-carboxylate isomerase (NCBI) 11, 289
RSP_1918 RSP_1918 Predicted alpha/beta hydrolase (NCBI) 11, 289
RSP_1925 RSP_1925 Transcriptional regulator, GntR family (NCBI) 63, 120
RSP_1958 RSP_1958 None 120, 319
RSP_1967 RSP_1967 hypothetical protein (NCBI) 11, 133
RSP_2019 RSP_2019 hypothetical protein (NCBI) 103, 120
RSP_2036 RSP_2036 putative ECF/RNA polymerase sigma factor protein (NCBI) 11, 105
RSP_2039 linC 2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase (2,5-DDOL dehydrogenase) (NCBI) 11, 120
RSP_2084 RSP_2084 hypothetical protein (NCBI) 11, 152
RSP_2574 RSP_2574 hypothetical protein (NCBI) 27, 120
RSP_2673 RSP_2673 Glucose dehydrogenase (NCBI) 120, 368
RSP_2870 aglK ABC alpha-glucoside transporter, ATPase subunit AglK (NCBI) 120, 265
RSP_2989 RSP_2989 hypothetical protein (NCBI) 11, 155
RSP_3205 RSP_3205 putative oxidoreductase subunit (NCBI) 120, 124
RSP_3385 RSP_3385 transcriptional regulator, LysR family (NCBI) 11, 220
RSP_3429 RSP_3429 hypothetical protein (NCBI) 11, 116
RSP_3937 RSP_3937 probable transposase protein, Y4bF (NCBI) 11, 132
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2039
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend