Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2307

probable manganese-dependent inorganic pyrophosphatase (NCBI)

CircVis
Functional Annotations (5)
Function System
Inorganic pyrophosphatase/exopolyphosphatase cog/ cog
inorganic diphosphatase activity go/ molecular_function
cytoplasm go/ cellular_component
manganese ion binding go/ molecular_function
Oxidative phosphorylation kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2307
(Mouseover regulator name to see its description)

RSP_2307 is regulated by 21 influences and regulates 0 modules.
Regulators for RSP_2307 (21)
Regulator Module Operator
RSP_0394 166 tf
RSP_0395 166 tf
RSP_0698 166 tf
RSP_0755 166 tf
RSP_1163 166 tf
RSP_1231 166 tf
RSP_1704 166 tf
RSP_1739 166 tf
RSP_2494 166 tf
RSP_2730 166 tf
RSP_2850 166 tf
RSP_2889 166 tf
RSP_2922 166 tf
RSP_0395 109 tf
RSP_0728 109 tf
RSP_1231 109 tf
RSP_1739 109 tf
RSP_2494 109 tf
RSP_2572 109 tf
RSP_2730 109 tf
RSP_3309 109 tf

Warning: RSP_2307 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
7938 1.50e+01 GtTTTCGGacatTT
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7939 3.20e+04 GAactGaAaccttgcCttCcgcC
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8052 4.30e+00 TTtccCGC
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8053 4.70e+02 AATTCAGACCTTTTCCTGA
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2307

RSP_2307 is enriched for 5 functions in 3 categories.
Enrichment Table (5)
Function System
Inorganic pyrophosphatase/exopolyphosphatase cog/ cog
inorganic diphosphatase activity go/ molecular_function
cytoplasm go/ cellular_component
manganese ion binding go/ molecular_function
Oxidative phosphorylation kegg/ kegg pathway
Module neighborhood information for RSP_2307

RSP_2307 has total of 48 gene neighbors in modules 109, 166
Gene neighbors (48)
Gene Common Name Description Module membership
RSP_0005 guaA GMP synthase (glutamine-hydrolyzing) (NCBI) 166, 258
RSP_0142 RSP_0142 FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor) (NCBI) 109, 369
RSP_0385 RSP_0385 Threonyl-tRNA synthetase, class IIa (NCBI) 166, 360
RSP_0403 RSP_0403 homoserine dehydrogenase (NCBI) 109, 299
RSP_0404 RSP_0404 fructose - 1,6 - bisphosphatase, GlpX-like (NCBI) 166, 203
RSP_0584 RSP_0584 Lysyl-tRNA synthetase (NCBI) 109, 279
RSP_0587 RSP_0587 Putative ABC transporter, fused ATPase subunits (NCBI) 109, 360
RSP_0655 RSP_0655 hypothetical protein (NCBI) 109, 369
RSP_0714 RSP_0714 hypothetical protein (NCBI) 109, 277
RSP_0778 ProS Prolyl-tRNA synthetase, class IIa (NCBI) 166, 258
RSP_0815 aspS Aspartyl-tRNA synthetase (NCBI) 166, 248
RSP_0827 RSP_0827 Ribosomal protein L25 (NCBI) 104, 166
RSP_0832 RSP_0832 Putative GTP-binding protein (NCBI) 166, 192
RSP_0968 mdh malate dehydrogenase (RefSeq) 25, 166
RSP_1068 argB acetylglutamate kinase (NCBI) 109, 192
RSP_1153 GutQ Sugar phosphate Isomerase (NCBI) 109, 192
RSP_1161 RSP_1161 3'-Phosphadenosine 5'-phosphosulfate 3'-phosphatase (NCBI) 109, 384
RSP_1212 argG ArgG, Argininosuccinate synthase (NCBI) 109, 166
RSP_1376 asd putative aspartate-semialdehyde dehydrogenase (NCBI) 166, 192
RSP_1575 sopT sulfate adenylyltransferase (NCBI) 109, 271
RSP_1761 pheT Phenylalanyl-tRNA synthetase beta chain (NCBI) 51, 166
RSP_1763 pheS Phenylalanyl-tRNA synthetase alpha subunit (NCBI) 109, 360
RSP_1795 serS Probable Seryl-tRNA synthetase (NCBI) 109, 192
RSP_1831 thrC Threonine synthase (NCBI) 109, 192
RSP_1849 RSP_1849 Aspartate kinase (NCBI) 109, 271
RSP_1856 glyQ Heterodimeric glycyl-transfer RNA synthetase (NCBI) 109, 258
RSP_2088 lepA GTP-binding elongation factor (NCBI) 51, 166
RSP_2221 purB Adenylosuccinate lyase (NCBI) 109, 369
RSP_2258 purC SAICAR synthetase (NCBI) 43, 109
RSP_2301 prsA Phosphoribosyl pyrophosphate synthetase (NCBI) 109, 203
RSP_2307 RSP_2307 probable manganese-dependent inorganic pyrophosphatase (NCBI) 109, 166
RSP_2453 RSP_2453 putative oxidoreductase (NAD/NADP dependent) (NCBI) 71, 166
RSP_2455 RSP_2455 putative Colicin V production protein, dedE (NCBI) 109, 299
RSP_2461 fabG 3-oxoacyl-(acyl-carrier protein) reductase (NCBI) 166, 195
RSP_2537 prfC Probable peptide chain release factor RF3 (NCBI) 72, 166
RSP_2615 RSP_2615 hypothetical protein (NCBI) 109, 345
RSP_2697 RSP_2697 hypothetical protein (NCBI) 166, 184
RSP_2698 RSP_2698 putative quinoprotein (NCBI) 166, 240
RSP_2699 RSP_2699 Probable GTP-binding protein (NCBI) 166, 271
RSP_2705 pyrH Uridylate kinase (NCBI) 109, 360
RSP_2706 frr Ribosome recycling factor (NCBI) 109, 360
RSP_2737 RSP_2737 Putative ssRNA endoribonuclease L-PSP (NCBI) 166, 345
RSP_2956 tktA Transketolase (NCBI) 166, 360
RSP_3349 RSP_3349 hypothetical protein (NCBI) 133, 166
RSP_4042 RSP_4042 Peptidyl-prolyl cis-trans isomerase, cyclophilin type (NCBI) 166, 345
RSP_4043 RSP_4043 Peptidylprolyl isomerase (NCBI) 162, 166
RSP_4044 pgk Phosphoglycerate kinase (NCBI) 166, 299
RSP_4045 fbaB Fructose-bisphosphate aldolase class I (NCBI) 166, 299
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_2307
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend