Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_2523 nuoI

Subunit of NADH-ubiquinone oxidoreductase (Complex I) that contains 2 Fe-S centers (NCBI)

CircVis
Functional Annotations (7)
Function System
Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) cog/ cog
electron transport go/ biological_process
NADH dehydrogenase (ubiquinone) activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
Oxidative phosphorylation kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
NuoI tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_2523
(Mouseover regulator name to see its description)

RSP_2523 is regulated by 29 influences and regulates 0 modules.
Regulators for RSP_2523 nuoI (29)
Regulator Module Operator
RSP_0402 269 tf
RSP_0443 269 tf
RSP_0547 269 tf
RSP_0958 269 tf
RSP_1055 269 tf
RSP_1739 269 tf
RSP_2324 269 tf
RSP_2346 269 tf
RSP_2606 269 tf
RSP_2719 269 tf
RSP_2963 269 tf
RSP_3324 269 tf
RSP_3341 269 tf
RSP_3436 269 tf
RSP_3731 269 tf
RSP_0623 361 tf
RSP_0768 361 tf
RSP_0794 361 tf
RSP_1014 361 tf
RSP_1055 361 tf
RSP_1712 361 tf
RSP_2533 361 tf
RSP_2681 361 tf
RSP_2801 361 tf
RSP_2838 361 tf
RSP_2850 361 tf
RSP_2950 361 tf
RSP_3125 361 tf
RSP_3341 361 tf

Warning: RSP_2523 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 2 motifs predicted.

Motif Table (2)
Motif Id e-value Consensus Motif Logo
8420 4.20e+01 aAGAAgaTcAc
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8421 3.70e+03 TGCGGcAGga
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_2523

RSP_2523 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I) cog/ cog
electron transport go/ biological_process
NADH dehydrogenase (ubiquinone) activity go/ molecular_function
iron-sulfur cluster binding go/ molecular_function
Oxidative phosphorylation kegg/ kegg pathway
Metabolic pathways kegg/ kegg pathway
NuoI tigr/ tigrfam
Module neighborhood information for RSP_2523

RSP_2523 has total of 38 gene neighbors in modules 269, 361
Gene neighbors (38)
Gene Common Name Description Module membership
RSP_0843 RSP_0843 Conserved protein containing sulfotransfer domain (NCBI) 87, 361
RSP_0844 RSP_0844 hypothetical protein (NCBI) 87, 361
RSP_0992 phaA/B pH adaption potassium efflux system, PhaA/B subunit (NCBI) 32, 361
RSP_0993 phaC pH adaption potassium efflux system, PhaC subunit (NCBI) 32, 361
RSP_0994 phaD pH adaption potassium efflux system, PhaD subunit (NCBI) 32, 361
RSP_0995 phaE pH adaption potassium efflux system, PhaE subunit (NCBI) 32, 361
RSP_0996 phaF pH adaption potassium efflux system, PhaF subunit (NCBI) 32, 361
RSP_0997 phaG pH adaption potassium efflux system, PhaG subunit (NCBI) 32, 361
RSP_1744 RSP_1744 CrcB-like protein (NCBI) 160, 361
RSP_1745 RSP_1745 Pseudouridine synthase (NCBI) 160, 361
RSP_1746 RSP_1746 hypothetical protein (NCBI) 326, 361
RSP_2342 gpt Xanthine-Guanine phosphoribosyltransferase (NCBI) 361, 369
RSP_2512 nuoA NADH dehydrogenase-ubiquinone oxidoreductase, chain A (NCBI) 228, 269
RSP_2513 nuoB NADH dehydrogenase-ubiquinone oxidoreductase, chain B (NCBI) 228, 269
RSP_2514 nuoC NADH dehydrogenase-ubiquinone oxidoreductase, chain C (NCBI) 228, 269
RSP_2515 nuoD NADH dehydrogenase-ubiquinone oxidoreductase, chain D (NCBI) 228, 269
RSP_2516 nuoE NADH dehydrogenase-ubiquinone oxidoreductase, chain E (NCBI) 228, 269
RSP_2517 RSP_2517 hypothetical protein (NCBI) 228, 269
RSP_2518 nuoF NADH dehydrogenase-ubiquinone oxidoreductase, chain F (NCBI) 228, 269
RSP_2519 RSP_2519 hypothetical protein (NCBI) 269, 372
RSP_2520 RSP_2520 hypothetical protein (NCBI) 228, 269
RSP_2521 nuoG NADH dehydrogenase-ubiquinone oxidoreductase, chain G (NCBI) 228, 269
RSP_2522 nuoH Respiratory-chain NADH dehydrogenase, subunit 1 (NCBI) 228, 269
RSP_2523 nuoI Subunit of NADH-ubiquinone oxidoreductase (Complex I) that contains 2 Fe-S centers (NCBI) 269, 361
RSP_2524 RSP_2524 possible carboxymuconolactone decarboxylase family protein (NCBI) 228, 269
RSP_2525 nuoJ NADH dehydrogenase I-ubiquinone oxidoreductase subunit 6 (chain J) (NCBI) 228, 269
RSP_2526 nuoK NADH-ubiquinone oxidoreductase subunit 11 or 4L (chain K) (NCBI) 228, 269
RSP_2527 nuoL NADH dehydrogenase I chain L (NCBI) 228, 269
RSP_2529 nuoM NADH dehydrogenase I chain M (NADH-ubiquinone oxidoreductase chain 13) (NCBI) 228, 269
RSP_2530 nuoN NADH dehydrogenase I chain N (NCBI) 228, 269
RSP_2532 birA putative biotin protein ligase (NCBI) 17, 361
RSP_2533 RSP_2533 Putative transcriptional regulator (NCBI) 361, 372
RSP_2534 RSP_2534 Predicted hydrolase of the metallo-beta-lactamase superfamily (NCBI) 84, 361
RSP_2777 RSP_2777 3-oxoacyl-(acyl-carrier-protein) synthase (NCBI) 87, 361
RSP_3710 RSP_3710 Monovalent cation/proton antiporter subunit/mnhG (NCBI) 311, 361
RSP_3711 RSP_3711 Multisubunit Na+/H+ antiporter MnhF subunit (NCBI) 311, 361
RSP_3714 RSP_3714 pH adaption potassium efflux system, phaC subunit (NCBI) 311, 361
RSP_3715 RSP_3715 pH adaption potassium efflux system, PhaB subunit (NCBI) 311, 361
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
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Comments for RSP_2523
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend