Organism : Rhodobacter sphaeroides 2.4.1 | Module List :
RSP_3431

periplasmic sensor signal transduction histidine kinase (NCBI)

CircVis
Functional Annotations (7)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for RSP_3431
(Mouseover regulator name to see its description)

RSP_3431 is regulated by 19 influences and regulates 0 modules.
Regulators for RSP_3431 (19)
Regulator Module Operator
RSP_0014 158 tf
RSP_0547 158 tf
RSP_0601 158 tf
RSP_0611 158 tf
RSP_1191 158 tf
RSP_1518 158 tf
RSP_1550 158 tf
RSP_1606 158 tf
RSP_1776 158 tf
RSP_2130 158 tf
RSP_2572 158 tf
RSP_2965 158 tf
RSP_3667 158 tf
RSP_3680 158 tf
RSP_3731 158 tf
RSP_0386 146 tf
RSP_1191 146 tf
RSP_2572 146 tf
RSP_3418 146 tf

Warning: RSP_3431 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
8012 3.20e+03 ATCTCg
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8013 5.00e+04 AATGCTGC
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8036 1.60e+00 TTGcAgaACgaAAAC
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8037 2.90e+01 CttTTCCCGA.ga.agGc
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for RSP_3431

RSP_3431 is enriched for 7 functions in 3 categories.
Enrichment Table (7)
Function System
Signal transduction histidine kinase cog/ cog
two-component sensor activity go/ molecular_function
ATP binding go/ molecular_function
signal transduction go/ biological_process
membrane go/ cellular_component
peptidyl-histidine phosphorylation go/ biological_process
Two-component system kegg/ kegg pathway
Module neighborhood information for RSP_3431

RSP_3431 has total of 30 gene neighbors in modules 146, 158
Gene neighbors (30)
Gene Common Name Description Module membership
RSP_0351 RSP_0351 D-threo-aldose 1-dehydrogenase (NCBI) 158, 216
RSP_0409 RSP_0409 hypothetical protein (NCBI) 134, 146
RSP_0608 RSP_0608 hypothetical protein (NCBI) 146, 216
RSP_0609 RSP_0609 hypothetical protein (NCBI) 146, 304
RSP_0619 RSP_0619 hypothetical protein (NCBI) 146, 188
RSP_0707 RSP_0707 hypothetical protein (NCBI) 158, 335
RSP_0796 cobQ cobyric acid synthase (NCBI) 95, 158
RSP_0917 RSP_0917 Inositol monophosphatase (NCBI) 158, 218
RSP_1321 fliR Flagellar biosynthesis pathway, component FliR (NCBI) 146, 340
RSP_1344 recF RecF protein (NCBI) 158, 218
RSP_1364 RSP_1364 hypothetical protein (NCBI) 146, 340
RSP_1540 RSP_1540 Predicted secreted hydrolase (NCBI) 125, 146
RSP_1541 RSP_1541 Putative ABC transporter, fused inner membrane subunits (NCBI) 125, 146
RSP_1755 RSP_1755 hypothetical protein (NCBI) 152, 158
RSP_1891 RSP_1891 Zinc-binding dehydrogenase (NCBI) 10, 158
RSP_2102 RSP_2102 hypothetical protein (NCBI) 110, 146
RSP_2146 trgB Tellurite resistance protein (NCBI) 158, 299
RSP_2222 RSP_2222 hypothetical protein (NCBI) 158, 171
RSP_2302 RSP_2302 putative low specificity L-threonine aldolase (NCBI) 54, 146
RSP_2314 RSP_2314 oxidoreductase, Chromogranin/secretogranin (NCBI) 158, 231
RSP_2972 RSP_2972 hypothetical protein (NCBI) 54, 158
RSP_2973 RSP_2973 Peroxiredoxin (NCBI) 158, 286
RSP_3140 RSP_3140 hypothetical protein (NCBI) 133, 158
RSP_3200 RSP_3200 Molecular chaperone, DnaK (NCBI) 146, 356
RSP_3310 RSP_3310 None 158, 210
RSP_3379 RSP_3379 hypothetical protein (NCBI) 133, 146
RSP_3390 RSP_3390 ABC Fe3+-siderophores transporter, ATPase subunit (NCBI) 146, 235
RSP_3418 RSP_3418 transcriptional regulator, AraC family (NCBI) 146, 340
RSP_3431 RSP_3431 periplasmic sensor signal transduction histidine kinase (NCBI) 146, 158
RSP_3779 RSP_3779 hypothetical protein (NCBI) 94, 146
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for RSP_3431
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend