Organism : Clostridium acetobutylicum | Module List :
CAC2154 flgE

Flagellar hook protein FlgE. (NCBI ptt file)

CircVis
Functional Annotations (6)
Function System
Flagellar hook protein FlgE cog/ cog
ciliary or flagellar motility go/ biological_process
motor activity go/ molecular_function
structural molecule activity go/ molecular_function
bacterial-type flagellum go/ cellular_component
FlgEFG_subfam tigr/ tigrfam
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for CAC2154
(Mouseover regulator name to see its description)

CAC2154 is regulated by 24 influences and regulates 0 modules.
Regulators for CAC2154 flgE (24)
Regulator Module Operator
CAC0078 96 tf
CAC0191 96 tf
CAC0289 96 tf
CAC0571 96 tf
CAC1467 96 tf
CAC2209 96 tf
CAC2306 96 tf
CAC2307 96 tf
CAC2773 96 tf
CAC3409 96 tf
CAC3729 96 tf
CAC0078 285 tf
CAC0191 285 tf
CAC1467 285 tf
CAC1578 285 tf
CAC1695 285 tf
CAC2060 285 tf
CAC2209 285 tf
CAC2254 285 tf
CAC2306 285 tf
CAC2307 285 tf
CAC2773 285 tf
CAC3271 285 tf
CAC3409 285 tf

Warning: CAC2154 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
6846 4.50e+01 GgGTatAAAag
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6847 2.90e+02 ACGATAATA
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7222 1.80e+00 aAgggtGcaaaaGt
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7223 2.10e+03 GCTTATCCTTTTACTTAAAGCC
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for CAC2154

CAC2154 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Flagellar hook protein FlgE cog/ cog
ciliary or flagellar motility go/ biological_process
motor activity go/ molecular_function
structural molecule activity go/ molecular_function
bacterial-type flagellum go/ cellular_component
FlgEFG_subfam tigr/ tigrfam
Module neighborhood information for CAC2154

CAC2154 has total of 31 gene neighbors in modules 96, 285
Gene neighbors (31)
Gene Common Name Description Module membership
CAC0117 121 protein cheY homolog (NCBI ptt file) 107, 285
CAC0152 CAC0152 Ribosomal-protein-alanine acetyltransferase (acetylating enzyme for N-terminal of ribosomal protein S5) (NCBI ptt file) 265, 285
CAC0304 motA Chemotaxis motility protein A, gene motA (NCBI ptt file) 93, 96
CAC0305 motB Chemotaxis motility protein B, gene motB (NCBI ptt file) 93, 96
CAC0311 CAC0311 PolyA polymerase (NCBI ptt file) 285, 355
CAC0384 licB PTS system, cellobiose-specific component BII (NCBI ptt file) 285, 324
CAC0385 CAC0385 Beta-glucosidase (NCBI ptt file) 285, 324
CAC0386 licC PTS cellobiose-specific component IIC (NCBI ptt file) 82, 285
CAC0387 CAC0387 Hypothetical protein (NCBI ptt file) 285, 324
CAC0542 CAC0542 Methyl-accepting chemotaxis protein (NCBI ptt file) 70, 285
CAC0741 CAC0741 Methyl-accepting chemotaxis protein (NCBI ptt file) 24, 285
CAC1233 chev Chemotaxis protein CheV ortholog (CheW-like adaptor domain and CheY-like reciever domain) (NCBI ptt file) 93, 285
CAC1433 CAC1433 Hypothetical protein (NCBI ptt file) 285, 319
CAC2154 flgE Flagellar hook protein FlgE. (NCBI ptt file) 96, 285
CAC2157 fliK Flagellar hook-length control protein fliK (NCBI ptt file) 96, 126
CAC2166 CAC2166 Nucleoside-diphosphate-sugar epimerase (NCBI ptt file) 96, 217
CAC2167 CAC2167 Flagellin family protein (NCBI ptt file) 96, 122
CAC2171 CAC2171 Predicted glycosyltransferase (NCBI ptt file) 96, 107
CAC2172 CAC2172 Predicted glycosyltransferase (NCBI ptt file) 28, 96
CAC2173 CAC2173 Glycosyltransferase (NCBI ptt file) 96, 107
CAC2175 CAC2175 Glycosyltransferase (NCBI ptt file) 96, 107
CAC2194 CAC2194 Predicted nucleoside-diphosphate sugar epimerase (NCBI ptt file) 96, 285
CAC2213 CAC2213 Hypothetical protein (NCBI ptt file) 96, 192
CAC2254 CAC2254 Response regulator (CheY-like receiver domain and HTH-type DNA-binding domain) (NCBI ptt file) 100, 285
CAC2338 CAC2338 Lysine decarboxylase (NCBI ptt file) 82, 285
CAC2488 CAC2488 Uncharacterized conserved protein, YTFE family (NCBI ptt file) 285, 367
CAC2619 CAC2619 Acyl-CoA thioesterase family protein (NCBI ptt file) 9, 96
CAC3271 CAC3271 Transcriptional regulator, AcrR family (NCBI ptt file) 223, 285
CAC3272 CAC3272 Possible surface protein, responsible for cell interaction; contains cell adhesion domain and ChW-repeats (NCBI ptt file) 285, 359
CAC3333 CAC3333 Uncharacterized conserved protein, related to pyruvate formate-lyase activating enzyme (NCBI ptt file) 265, 285
CAC3388 CAC3388 Methyl-accepting chemotaxis protein (NCBI ptt file) 48, 96
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for CAC2154
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend