Organism : Geobacter sulfurreducens | Module List :
GSU1238

iron-sulfur cluster-binding protein (VIMSS)

CircVis
Functional Annotations (6)
Function System
Fe-S-cluster-containing hydrogenase components 1 cog/ cog
electron transport go/ biological_process
formate dehydrogenase (NAD+) activity go/ molecular_function
electron carrier activity go/ molecular_function
formate dehydrogenase complex go/ cellular_component
iron-sulfur cluster binding go/ molecular_function
GeneModule member RegulatorRegulator MotifMotif

Cytoscape Web
Regulation information for GSU1238
(Mouseover regulator name to see its description)

GSU1238 is regulated by 24 influences and regulates 0 modules.
Regulators for GSU1238 (24)
Regulator Module Operator
GSU0266 252 tf
GSU0735 252 tf
GSU1115 252 tf
GSU2581 252 tf
GSU2831 252 tf
GSU2868 252 tf
GSU3217 252 tf
GSU3387 252 tf
GSU0164 72 tf
GSU0191 72 tf
GSU0205 72 tf
GSU0254 72 tf
GSU0366 72 tf
GSU0721 72 tf
GSU0735 72 tf
GSU0811 72 tf
GSU0812 72 tf
GSU1268 72 tf
GSU1270 72 tf
GSU1410 72 tf
GSU1483 72 tf
GSU2817 72 tf
GSU2987 72 tf
GSU3127 72 tf

Warning: GSU1238 Does not regulate any modules!

Motif information (de novo identified motifs for modules)

There are 4 motifs predicted.

Motif Table (4)
Motif Id e-value Consensus Motif Logo
2304 2.70e+02 TttGttGtagAaa
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2305 1.80e-03 cGCcTTTttTtTT
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2664 8.40e-01 tAaacACCaaaaccg.AtgaA
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2665 2.00e-01 cC..cCCgC.cagAa.g.cG
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Motif Help

Transcription factor binding motifs help to elucidate regulatory mechanism. cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. De novo predicted motifs for each module are listed in the module page as motif logo images along with associated prediction statistics (e-values). The main module page also shows the location of these motifs within the upstream sequences of the module member genes.

Motifs of interest can be broadcasted to RegPredict (currently only available for Desulfovibrio vulgaris Hildenborough) in order to compare conservation in similar species. This integrated motif prediction and comparative analysis provides an additional checkpoint for regulatory motif prediction confidence.

Motif e-value: cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. Motif e-value is an indicative of the motif co-occurences between the members of the module.Smaller e-values are indicative of significant sequence motifs. Our experience showed that e-values smaller than 10 are generally indicative of significant motifs.

Functional Enrichment for GSU1238

GSU1238 is enriched for 6 functions in 3 categories.
Enrichment Table (6)
Function System
Fe-S-cluster-containing hydrogenase components 1 cog/ cog
electron transport go/ biological_process
formate dehydrogenase (NAD+) activity go/ molecular_function
electron carrier activity go/ molecular_function
formate dehydrogenase complex go/ cellular_component
iron-sulfur cluster binding go/ molecular_function
Module neighborhood information for GSU1238

GSU1238 has total of 43 gene neighbors in modules 72, 252
Gene neighbors (43)
Gene Common Name Description Module membership
GSU0123 GSU0123 nickel-dependent hydrogenase, small subunit (VIMSS) 96, 252
GSU0471 GSU0471 sensor histidine kinase (VIMSS) 252, 268
GSU0543 GSU0543 outer membrane lipoprotein, Slp family, putative (VIMSS) 229, 252
GSU0544 GSU0544 conserved hypothetical protein (NCBI) 229, 252
GSU0545 GSU0545 membrane protein, putative (VIMSS) 226, 252
GSU0546 GSU0546 peptidyl-tRNA hydrolase domain protein (NCBI) 69, 252
GSU0547 mutS2 MutS2 family protein (NCBI) 252, 287
GSU0722 GSU0722 hypothetical protein (VIMSS) 72, 150
GSU0791 GSU0791 conserved hypothetical protein (VIMSS) 107, 252
GSU0795 GSU0795 Rhodanese-like domain protein (VIMSS) 173, 252
GSU0868 GSU0868 conserved hypothetical protein TIGR00159 (NCBI) 64, 72
GSU0939 GSU0939 nitrogen regulatory protein P-II, putative (VIMSS) 64, 72
GSU0940 GSU0940 ammonium transporter (VIMSS) 64, 72
GSU0941 GSU0941 sensor histidine kinase, putative (VIMSS) 64, 72
GSU0967 GSU0967 membrane protein, putative (VIMSS) 32, 252
GSU0968 GSU0968 hypothetical protein (VIMSS) 64, 72
GSU1101 GSU1101 sensory box histidine kinase (VIMSS) 252, 287
GSU1235 GSU1235 conserved hypothetical protein (VIMSS) 72, 202
GSU1236 GSU1236 conserved hypothetical protein (NCBI) 72, 202
GSU1237 GSU1237 pyridine nucleotide-disulphide oxidoreductase family protein (NCBI) 4, 72
GSU1238 GSU1238 iron-sulfur cluster-binding protein (VIMSS) 72, 252
GSU1297 GSU1297 ISGsu2, transposase (VIMSS) 72, 164
GSU1834 GSU1834 peptidase, M50 family (NCBI) 72, 205
GSU1835 glnA glutamine synthetase, type I (NCBI) 4, 72
GSU1836 GSU1836 nitrogen regulatory protein P-II (VIMSS) 4, 72
GSU2174 GSU2174 conserved hypothetical protein (VIMSS) 229, 252
GSU2463 GSU2463 glycosyl transferase, group 1 family protein (NCBI) 165, 252
GSU2480 kdpA potassium-transporting ATPase, A subunit (NCBI) 96, 252
GSU2636 GSU2636 alpha-amylase family protein (VIMSS) 195, 252
GSU2709 GSU2709 hypothetical protein (VIMSS) 72, 151
GSU2762 glpK glycerol kinase (NCBI) 4, 252
GSU2784 GSU2784 hypothetical protein (VIMSS) 6, 252
GSU2798 GSU2798 acetyltransferase, GNAT family (NCBI) 64, 72
GSU2803 GSU2803 dinitrogenase iron-molybdenum cofactor family protein (NCBI) 64, 72
GSU2804 GSU2804 ferredoxin family protein (VIMSS) 64, 72
GSU2805 nifX nitrogenase molybdenum-iron cofactor biosynthesis protein NifX (NCBI) 64, 72
GSU2816 GSU2816 sensory box histidine kinase/response regulator (VIMSS) 72, 100
GSU2819 nifK nitrogenase molybdenum-iron protein, beta subunit (NCBI) 64, 72
GSU2822 GSU2822 response regulator (VIMSS) 64, 72
GSU2961 modB molybdenum ABC transporter, permease protein modB (NCBI) 64, 72
GSU3143 GSU3143 peptidyl-prolyl cis-trans isomerase, FKBP-type, putative (NCBI) 64, 72
GSU3153 GSU3153 iron-sulfur cluster-binding protein (VIMSS) 252, 266
GSU3189 GSU3189 hypothetical protein (VIMSS) 178, 252
Gene Page Help

Network Tab

If the gene is associated with a module(s), its connection to given modules along with other members of that module are shown as network by using CytoscapeWeb. In this view, each green colored circular nodes represent module member genes, purple colored diamonds represent module motifs and red triangles represent regulators. Each node is connected to module (Bicluster) via edges. This representation provides quick overview of all genes, regulators and motifs for modules. It also allows one to see shared genes/motifs/regulators among diferent modules.

Network representation is interactive. You can zoom in/out and move nodes/edges around. Clicking on a node will open up a window to give more details. For genes, Locus tag, organism, genomic coordinates, NCBI gene ID, whether it is transcription factor or not and any associated functional information will be shown. For regulators, number of modules are shown in addition to gene details. For motifs, e-value, consensus sequence and sequence logo will be shown. For modules, expression profile plot, motif information, functional associations and motif locations for each member of the module will be shown.
You can pin information boxes by using button in the box title and open up additional ones on the same screen for comparative analysis.

Regulation Tab

Regulation tab for each gene includes regulatory influences such as environmental factors or transcription factors or their combinations identified by regulatory network inference algorithms.

If the gene is a member of a module, regulators influencing that module are also considered to regulate the gene. Regulators table list total number of regulatory influences, regulators, modules and type of the influence.

You can see description of the regulator inside the tooltip when you mouseover. In certain cases the regulatory influence is predicted to be the result of the combination of two influences. These are indicated as combiner in the column labeled "Operator".

For transcription factors, an additional table next to regulator table will be show. This table show modules that are influenced by the transcription factor.

Motifs Tab

Network inference algorithm uses de novo motif prediction for assigning genes to modules. If there are any motifs identified in the upstream region of a gene, the motif will be shown here. For each motif sequence logo, consensus and e-value will be shown.

Functions Tab

Identification of functional enrichment for the module members is important in associating predicted motifs and regulatory influences with pathways. As described above, the network inference pipeline includes a functional enrichment module by which hypergeometric p-values are used to identify over representation of functional ontology terms among module members.

Network Portal presents functional ontologies from KEGG, GO, TIGRFAM, and COG as separate tables that include function name, type, corrected and uncorrected hypergeometric p-values, and the number of genes assigned to this category out of total number of genes in the module.

Module Members Tab

Identity of gene members in a module may help to identify potential interactions between different functional modules. Therefore, neighbor genes that share the same module(s) with gene under consideration are shown here. For each memebr, gene name, description and modules that contain it are listed.

Help Tab

This help page. More general help can be accessed by clicking help menu in the main navigation bar.

Social Tab

Network Portal is designed to promote collaboration through social interactions. Therefore interested researchers can share information, questions and updates for a particular gene.

Users can use their Disqus, Facebook, Twitter or Google accounts to connect to this page (We recommend Google). Each module and gene page includes comments tab that lists history of the interactions for that gene. You can browse the history, make updates, raise questions and share these activities with social web.

In the next releases of the network portal, we are planning to create personal space for each user where you can share you space that contains all the analysis steps you did along with relevant information.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend
Comments for GSU1238
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Gene Help

Overview

Gene landing pages present genomic, functional, and regulatory information for individual genes. A circular visualization displays connections between the selected gene and genes in the same modules, with as edges drawn between the respective coordinates of the whole genome.

The gene page also lists functional ontology assignments, module membership, and motifs associated with these modules. Genes in the network inherit regulatory influences from the modules to which they belong. Therefore, the regulatory information for each gene is a collection of all regulatory influences on these modules. These are listed as a table that includes influence name, type, and target module. If the gene is a transcription factor, its target modules are also displayed in a table that provides residual values and number of genes.

CircVis

Our circular module explorer is adapted from visquick originally developed by Dick Kreisberg of Ilya Shmulevich lab at ISB for The Cancer Genome Atlas. We use simplified version of visquick to display distribution of module members and their interactions across the genome. This view provides summary of regulation information for a gene. The main components are;
  • 1. All genomic elements for the organism are represented as a circle and each element is separated by black tick marks. In this example chromosome and pDV represent main chromosome and plasmid for D. vulgaris Hildenborough, respectively.
  • 2. Source gene
  • 3. Target genes (other module members)
  • 4. Interactions between source and target genes for a particular module
  • 5. Module(s) that source gene and target genes belong to
  • 6. Visualisation legend