Rv0009 Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0009 ppiA Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) CDS 12468 13016 + 549 182 FALSE

Rv0009 (Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8)) is predicted to be co-regulated in modules bicluster_0100 with residual 0.55 and bicluster_0505 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 310.00 for bicluster_0100 and 0.00 and 0.00 for bicluster_0505 respectively.

These modules are enriched for following go terms: oxidoreductase activity, oxidoreductase activity, acting on NAD(P... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0100
e.value: 
130
Motif Bicluster: 
e.value: 
310
Motif Bicluster: 
0.55
bicluster_0505
e.value: 
0.000016
Motif Bicluster: 
e.value: 
0.00038
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
Probable peptidyl-prolyl cis-trans isomerase A iron-regulated peptidyl-prolyl cis-trans isomerase A
Operon # Operon
6
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607151 NP_214523.1 Run
GO:0003755

peptidyl-prolyl cis-trans isomerase activity

peptidyl-prolyl cis-trans isomerase activity

Details: 
Catalysis of the reaction: peptidyl-proline (omega=180) = peptidyl-proline (omega=0).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0010039

response to iron ion

response to iron ion

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of an iron ion stimulus.
GO Category: 
biological_process
8
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.27

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: