Rv0010c Possible membrane protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0010c Possible membrane protein CDS 13133 13558 - 426 141 FALSE

Rv0010c (Possible membrane protein) is predicted to be co-regulated in modules bicluster_0329 with residual 0.54 and bicluster_0474 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 1.10 for bicluster_0329 and 0.24 and 18,000.00 for bicluster_0474 respectively.

These modules are enriched for following go terms: inositol metabolic process, inositol biosynthetic process, polyol biosynthetic process, acyl-CoA dehydrogenase activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
ESX-1 secreted protein regulator EspR
No -68 0.48 0.0771699 CDS
cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases
No -71 -0.06 0.946187 CDS
\Transcriptional regulator, TetR family\\\""
No -87 0.13 0.944941 CDS
Motif 1 Motif 2 Residual
bicluster_0329
e.value: 
0.00000000023
Motif Bicluster: 
e.value: 
1.1
Motif Bicluster: 
0.54
bicluster_0474
e.value: 
0.24
Motif Bicluster: 
e.value: 
18000
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0010c_MT0013
Operon # Operon
7
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607152 NP_214524.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.720000 1.16

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: