Rv0013 Anthranilate synthase, amidotransferase component (EC 220.127.116.11) @ Para-aminobenzoate synthase, amidotransferase component (EC 18.104.22.168)
|Symbol||Product||Feature Type||Start||End||Strand||Length||AA Length||is TF|
|Rv0013||trpG||Anthranilate synthase, amidotransferase component (EC 22.214.171.124) @ Para-aminobenzoate synthase,...||CDS||14914||15612||+||699||232||FALSE|
Rv0013 (Anthranilate synthase, amidotransferase component (EC 126.96.36.199) @ Para-aminobenzoate synthase, amidotransferase component (EC 188.8.131.52)) is predicted to be co-regulated in modules bicluster_0018 with residual 0.62 and bicluster_0079 with residual 0.57.
This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.55 and 25.00 for bicluster_0018 and 0.70 and 11.00 for bicluster_0079 respectively.
These modules are enriched for following go terms: potassium ion transport.
This gene is found to be for growth on cholesterol.
Mutant available?: Yes
|Product (LegacyBRC)||Product (RefSeq)|
|POSSIBLE ANTHRANILATE SYNTHASE COMPONENT II TRPG [GLUTAMINE AMIDOTRANSFERASE]||para-aminobenzoate synthase component II|
|BioCyc Gene Page||Cellular Overview Map|
|No results were found|
|t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.
reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.