Rv0050 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0050 ponA1 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) CDS 53663 55699 + 2 037 678 FALSE

Rv0050 (Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)) is predicted to be co-regulated in modules bicluster_0105 with residual 0.56 and bicluster_0466 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,200.00 and 5,800.00 for bicluster_0105 and 0.57 and 9,200.00 for bicluster_0466 respectively.

These modules are enriched for following go terms: potassium ion transport, ion transmembrane transporter activity, substrate-specific transmembrane transpo..., substrate-specific transporter activity, transmembrane transporter activity, cation transmembrane transporter activit....

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE BIFUNCTIONAL PENICILLIN-BINDING PROTEIN 1A_1B PONA1 [MUREIN POLYMERASE] [PBP1]: PENICILLIN-INSENSITIVE TRANSGLYCOSYLASE [PEPTIDOGLYCAN TGASE] + PENICILLIN-SENSITIVE TRANSPEPTIDASE [DD-TRANSPEPTIDASE] bifunctional penicillin-binding protein 1A/1B
Operon # Operon
34 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Peptidoglycan glycosyltransferase Peptidoglycan biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116685 YP_177687.1 Run
GO:0008955

peptidoglycan glycosyltransferase activity

peptidoglycan glycosyltransferase activity

Details: 
Catalysis of the reaction: [GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphosphoundecaprenol + GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1,4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.
GO Category: 
molecular_function
5
Total items in this category:  
GO:0008658

penicillin binding

penicillin binding

Details: 
Interacting selectively and non-covalently with penicillin, any antibiotic that contains the condensed beta-lactamthiazolidine ring system.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0030288

outer membrane-bounded periplasmic space

outer membrane-bounded periplasmic space

Details: 
The region between the inner (cytoplasmic or plasma) membrane and outer membrane of organisms with two membranes such as Gram negative bacteria. These periplasmic spaces are relatively thick and contain a thin cell wall.
GO Category: 
cellular_component
1
Total items in this category:  
GO:0052572

response to host immune response

response to host immune response

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting the immune response of the host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
62
Total items in this category:  
GO:0071456

cellular response to hypoxia

cellular response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
7
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.850000 2.86

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: