Rv0062 Endoglucanase A precursor (EC 3.2.1.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0062 celA1 Endoglucanase A precursor (EC 3.2.1.4) CDS 65552 66694 + 1 143 380 FALSE

Rv0062 (Endoglucanase A precursor (EC 3.2.1.4)) is predicted to be co-regulated in modules bicluster_0125 with residual 0.48 and bicluster_0557 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 28.00 for bicluster_0125 and 7,700.00 and 9,400.00 for bicluster_0557 respectively.

These modules are enriched for following go terms: transport, establishment of localization, localization, transporter activity chromosome segregation, cell cycle, cell division.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.301 65771 65552
Product (LegacyBRC) Product (RefSeq)
POSSIBLE CELLULASE CELA1 [ENDOGLUCANASE] [ENDO-14-BETA-GLUCANASE] [FI-CMCASE] [CARBOXYMETHYL CELLULASE] endo-1,4-beta-glucanase
Operon # Operon
40
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Cellulase Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116687 YP_177689.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0008810

cellulase activity

cellulase activity

Details: 
Catalysis of the endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.130000 0.99

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: