Rv0085 Hydrogenase-4 component E (EC 1.-.-.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0085 hycP Hydrogenase-4 component E (EC 1.-.-.-) CDS 93289 93951 + 663 220 FALSE

Rv0085 (Hydrogenase-4 component E (EC 1.-.-.-)) is predicted to be co-regulated in modules bicluster_0143 with residual 0.53 and bicluster_0391 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 130.00 for bicluster_0143 and 0.00 and 0.00 for bicluster_0391 respectively.

These modules are enriched for following go terms: cellular respiration, energy derivation by oxidation of organi..., generation of precursor metabolites and ..., oxidation-reduction process, NADH dehydrogenase activity, oxidoreductase activity, acting on NAD(P..., oxidoreductase activity, acting on NAD(P... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0085_MT0092 hydrogenase HycP
Operon # Operon
56 - - - - - - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607227 NP_214599.1 Run
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.290000 0.65

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: