Rv0126 Trehalose synthase (EC 5.4.99.16)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0126 treS Trehalose synthase (EC 5.4.99.16) CDS 152324 154129 + 1 806 601 FALSE

Rv0126 (Trehalose synthase (EC 5.4.99.16)) is predicted to be co-regulated in modules bicluster_0081 with residual 0.55 and bicluster_0493 with residual 0.66.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.53 and 19,000.00 for bicluster_0081 and 0.02 and 25.00 for bicluster_0493 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15063 MT0134 2609
Product (LegacyBRC) Product (RefSeq)
TREHALOSE SYNTHASE TRES trehalose synthase TRES
Operon # Operon
86
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Maltose alpha-D-glucosyltransferase Starch and sucrose metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Starch and sucrose metabolism

20
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607268 NP_214640.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0005978

glycogen biosynthetic process

glycogen biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of glycogen, a polydisperse, highly branched glucan composed of chains of D-glucose residues.
GO Category: 
biological_process
3
Total items in this category:  
GO:0005992

trehalose biosynthetic process

trehalose biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of trehalose, a disaccharide isomeric with sucrose and obtained from certain lichens and fungi.
GO Category: 
biological_process
2
Total items in this category:  
GO:0016161

beta-amylase activity

beta-amylase activity

Details: 
Catalysis of the reaction: (1,4-alpha-D-glucosyl)(n+1) + H2O = (1,4-alpha-D-glucosyl)(n-1) + alpha-maltose. This reaction is the hydrolysis of 1,4-alpha-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0047471

maltose alpha-D-glucosyltransferase activity

maltose alpha-D-glucosyltransferase activity

Details: 
Catalysis of the reaction: maltose = trehalose.
GO Category: 
molecular_function
1
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.200000 2.68

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: