Rv0137c Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0137c msrA Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11) CDS 164712 165260 - 549 182 FALSE

Rv0137c (Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)) is predicted to be co-regulated in modules bicluster_0099 with residual 0.60 and bicluster_0140 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 5.70 and 470.00 for bicluster_0099 and 0.00 and 0.00 for bicluster_0140 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
Repressed 10 -2.92 8.87e-29 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0099
e.value: 
5.7
Motif Bicluster: 
e.value: 
470
Motif Bicluster: 
0.60
bicluster_0140
e.value: 
0.0000061
Motif Bicluster: 
e.value: 
0.000019
Motif Bicluster: 
0.62
Product (LegacyBRC) Product (RefSeq)
Peptide methionine sulfoxide reductase msrA methionine sulfoxide reductase A
Operon # Operon
97
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607279 NP_214651.1 Run
GO:0033744

L-methionine-(S)-S-oxide reductase activity

L-methionine-(S)-S-oxide reductase activity

Details: 
Catalysis of the reaction: L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0006979

response to oxidative stress

response to oxidative stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of oxidative stress, a state often resulting from exposure to high levels of reactive oxygen species, e.g. superoxide anions, hydrogen peroxide (H2O2), and hydroxyl radicals.
GO Category: 
biological_process
13
Total items in this category:  
GO:0008113

peptide-methionine-(S)-S-oxide reductase activity

peptide-methionine-(S)-S-oxide reductase activity

Details: 
Catalysis of the reactions: peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (S)-S-oxide + thioredoxin, and L-methionine + thioredoxin disulfide + H2O = L-methionine (S)-S-oxide + thioredoxin.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0051409

response to nitrosative stress

response to nitrosative stress

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a nitrosative stress stimulus. Nitrosative stress is a state often resulting from exposure to high levels of nitric oxide (NO) or the highly reactive oxidant peroxynitrite, which is produced following interaction of NO with superoxide anions.
GO Category: 
biological_process
14
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.160000 0.74

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: