Rv0144 Transcriptional regulator, TetR family

Product Feature Type Start End Strand Length AA Length is TF
Rv0144 Transcriptional regulator, TetR family CDS 170284 171126 + 843 280 FALSE

Rv0144 (Transcriptional regulator, TetR family) is predicted to be co-regulated in modules bicluster_0101 with residual 0.62 and bicluster_0315 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.03 for bicluster_0101 and 0.00 and 0.00 for bicluster_0315 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.061 201 170485 -3 170482 170284
Product (LegacyBRC) Product (RefSeq)
Operon # Operon
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics


Not assigned Not assigned
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607286 NP_214658.1 Run



The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
Total items in this category:  

plasma membrane

plasma membrane

The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.560000 1.23

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00294292
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 11.64 U
D3I 3 3 3.83 11.77 I
D3U 3 3 3.83 11.41 U
D5I 9 5 6.00 12.38 I
D5U 17 5 6.00 12.14 U
D7I 18 7 8.14 12.41 I
D7U 19 7 8.14 11.32 U
D14I 4 14 15.63 10.87 I
D14U 4 14 15.63 10.07 U
D17I 3 17 19.15 10.20 I
D17U 3 17 19.15 9.43 U
D21I 4 21 23.23 10.63 I
D21U 4 21 23.23 9.81 U
D24I 3 24 26.60 10.24 I
D24U 3 24 26.60 9.21 U
D28I 4 28 30.61 10.47 I
D28U 4 28 30.61 9.79 U