Rv0154c Butyryl-CoA dehydrogenase (EC 1.3.99.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0154c fadE2 Butyryl-CoA dehydrogenase (EC 1.3.99.2) CDS 181987 183198 - 1 212 403 FALSE

Rv0154c (Butyryl-CoA dehydrogenase (EC 1.3.99.2)) is predicted to be co-regulated in modules bicluster_0098 with residual 0.64 and bicluster_0314 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0098 and 0.00 and 0.00 for bicluster_0314 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Primary TSS Re-Annotated Start Tuberculist Annotated Start
-1.314 183210 183210 183198
Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17960 MT0163 1012
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, PadR family
Repressed 8 -0.98 0.000000177 Primary.TSS
Repressed 60 -0.92 0.000000501 Primary.TSS
Transcriptional regulator, AsnC family
No 65 -0.4 0.143449 Primary.TSS
No 19 -0.05 0.937133 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0098
e.value: 
0
Motif Bicluster: 
e.value: 
3.3e-26
Motif Bicluster: 
0.64
bicluster_0314
e.value: 
2e-19
Motif Bicluster: 
e.value: 
0.0000016
Motif Bicluster: 
0.57
Product (LegacyBRC) Product (RefSeq)
PROBABLE ACYL-CoA DEHYDROGENASE FADE2 acyl-CoA dehydrogenase FADE2
Operon # Operon
110 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid metabolism

51
Total items in this category:  

KEGG

Valine, leucine and isoleucine degradation

60
Total items in this category:  

KEGG

beta-Alanine metabolism

37
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607296 NP_214668.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.41

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: