Rv0155 NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0155 pntAa NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2) CDS 183622 184722 + 1 101 366 FALSE

Rv0155 (NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)) is predicted to be co-regulated in modules bicluster_0100 with residual 0.55 and bicluster_0381 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 130.00 and 310.00 for bicluster_0100 and 0.18 and 6,300.00 for bicluster_0381 respectively.

These modules are enriched for following go terms: oxidoreductase activity, oxidoreductase activity, acting on NAD(P... inorganic anion transport, secondary active transmembrane transport....

This gene is found to be for growth on cholesterol.

Mutant available?:

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Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
No -94 -0.17 0.544837 CDS
No 22 0.19 0.999609 CDS
No -31 0.25 0.388061 CDS
Motif 1 Motif 2 Residual
bicluster_0100
e.value: 
130
Motif Bicluster: 
e.value: 
310
Motif Bicluster: 
0.55
bicluster_0381
e.value: 
0.18
Motif Bicluster: 
e.value: 
6300
Motif Bicluster: 
0.54
Product (LegacyBRC) Product (RefSeq)
PROBABLE NAD[P] TRANSHYDROGENASE [SUBUNIT ALPHA] PNTAA [FIRST PART CATALYTIC PART] [PYRIDINE NUCLEOTIDE TRANSHYDROGENASE SUBUNIT ALPHA] [NICOTINAMIDE NUCLEOTIDE TRANSHYDROGENASE SUBUNIT ALPHA] NAD(P) transhydrogenase subunit alpha
Operon # Operon
111 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Nicotinate and nicotinamide metabolism

16
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607297 NP_214669.1 Run
GO:0008750

NAD(P)+ transhydrogenase (AB-specific) activity

NAD(P)+ transhydrogenase (AB-specific) activity

Details: 
Catalysis of the reaction: NADPH + H+ + NAD+ = NADP+ + NADH + H+. The reaction is A-specific (i.e. the pro-R hydrogen is transferred from the 4-position of reduced nicotinamide cofactor) with respect to NAD+ and B-specific (i.e. the pro-S hydrogen is transferred) with respect to NADP+.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.140000 0.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: