Rv0182c RNA polymerase factor sigma-70

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0182c sigG RNA polymerase factor sigma-70 CDS 213028 214140 - 1 113 370 FALSE

Rv0182c (RNA polymerase factor sigma-70) is predicted to be co-regulated in modules bicluster_0048 with residual 0.53 and bicluster_0241 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.40 and 37.00 for bicluster_0048 and 5,500.00 and 8,700.00 for bicluster_0241 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.904 114 214026 553 214579 214026 214140
Product (LegacyBRC) Product (RefSeq)
RNA polymerase sigma factor RNA polymerase factor sigma-70
Operon # Operon
124 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

RNA polymerase

17
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607323 NP_214696.1 Run
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0009409

response to cold

response to cold

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a cold stimulus, a temperature stimulus below the optimal temperature for that organism.
GO Category: 
biological_process
3
Total items in this category:  
GO:0009415

response to water stimulus

response to water stimulus

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus reflecting the presence, absence, or concentration of water.
GO Category: 
biological_process
4
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.000000 0.90

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.58015
p-value INH: 0.231599
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 10.91 U
D3I 3 3 3.83 10.51 I
D3U 3 3 3.83 9.67 U
D5I 9 5 6.00 11.29 I
D5U 17 5 6.00 11.28 U
D7I 18 7 8.14 11.05 I
D7U 19 7 8.14 10.95 U
D14I 4 14 15.63 9.51 I
D14U 4 14 15.63 10.24 U
D17I 3 17 19.15 9.33 I
D17U 3 17 19.15 10.49 U
D21I 4 21 23.23 9.14 I
D21U 4 21 23.23 10.64 U
D24I 3 24 26.60 8.76 I
D24U 3 24 26.60 10.71 U
D28I 4 28 30.61 8.19 I
D28U 4 28 30.61 10.91 U