Rv0189c Dihydroxy-acid dehydratase (EC 4.2.1.9)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0189c ilvD Dihydroxy-acid dehydratase (EC 4.2.1.9) CDS 219996 221723 - 1 728 575 FALSE

Rv0189c (Dihydroxy-acid dehydratase (EC 4.2.1.9)) is predicted to be co-regulated in modules bicluster_0430 with residual 0.49 and bicluster_0569 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,000.00 and 5,600.00 for bicluster_0430 and 500.00 and 460.00 for bicluster_0569 respectively.

These modules are enriched for following go terms: primary metabolic process, cellular metabolic process, cellular process, organic substance metabolic process purine-nucleoside phosphorylase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Dihydroxy-acid dehydratase dihydroxy-acid dehydratase
Operon # Operon
130
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Pantothenate and CoA biosynthesis

18
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607330 NP_214703.1 Run
GO:0004160

dihydroxy-acid dehydratase activity

dihydroxy-acid dehydratase activity

Details: 
Catalysis of the reaction: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: