Rv0208c tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0208c yggH tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33) CDS 248115 248906 - 792 263 FALSE

Rv0208c (tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)) is predicted to be co-regulated in modules bicluster_0231 with residual 0.47 and bicluster_0408 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.10 and 6,500.00 for bicluster_0231 and 0.01 and 0.62 for bicluster_0408 respectively.

These modules are enriched for following go terms: fatty acid synthase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR Re-Annotated Start Tuberculist Annotated Start
-0.058 18 248888 180 248708 248906
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 15
ChipSeq TF Differential Expression Distance Expression pvalue Type
HTH-type transcriptional regulator
No -6 0.26 0.18753 Internal.TSS
Repressed 6 -0.88 0.0000000305 Internal.TSS
Two component DNA binding Transcriptional regulatory protein TcrA
No -1 0.08 0.841587 Internal.TSS
TetR/ACRR family transcriptional regulator
No -8 -0.02 0.99224 Internal.TSS
DNA-binding response regulator
No 7 -0.27 0.0964239 Internal.TSS
transcriptional regulator, ArsR family
Induced -14 1.24 1.11e-31 Internal.TSS
TetR/ACRR family transcriptional regulator
No -26 -0.02 0.99224 CDS
DNA-binding response regulator mtrA
No 29 0.35 0.30427 Internal.TSS
Histone protein Lsr2
No 17 0 0.998523 Internal.TSS
Transcriptional regulator, TetR family
No 29 -0.34 0.134752 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0231
e.value: 
3.1
Motif Bicluster: 
e.value: 
6500
Motif Bicluster: 
0.47
bicluster_0408
e.value: 
0.011
Motif Bicluster: 
e.value: 
0.62
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
tRNA [guanine-N[7]-]-methyltransferase tRNA (guanine-N(7)-)-methyltransferase
Operon # Operon
144 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607349 NP_214722.1 Run
GO:0008176

tRNA (guanine-N7-)-methyltransferase activity

tRNA (guanine-N7-)-methyltransferase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N7-methylguanine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.240000 0.14

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: