Rv0220 Esterase LipC

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0220 lipC Esterase LipC CDS 262812 264023 + 1 212 403 FALSE

Rv0220 (Esterase LipC) is predicted to be co-regulated in modules bicluster_0210 with residual 0.48 and bicluster_0225 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 29.00 for bicluster_0210 and 0.00 and 0.00 for bicluster_0225 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18060 MT0230 1305
Product (LegacyBRC) Product (RefSeq)
PROBABLE ESTERASE LIPC esterase LipC
Operon # Operon
152 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Bisphenol degradation

35
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607361 NP_214734.1 Run
GO:0004806

triglyceride lipase activity

triglyceride lipase activity

Details: 
Catalysis of the reaction: triacylglycerol + H2O = diacylglycerol + a fatty acid anion.
GO Category: 
molecular_function
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.220000 0.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: