Rv0223c Aldehyde dehydrogenase (EC 1.2.1.3)

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0223c Aldehyde dehydrogenase (EC 1.2.1.3) CDS 266301 267764 - 1 464 487 FALSE

Rv0223c (Aldehyde dehydrogenase (EC 1.2.1.3)) is predicted to be co-regulated in modules bicluster_0122 with residual 0.57 and bicluster_0147 with residual 0.43.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.10 and 6.50 for bicluster_0122 and 160.00 and 4,800.00 for bicluster_0147 respectively.

These modules are enriched for following go terms: cellular protein modification process, protein modification process, phosphorylation, protein kinase activity .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 17:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17946 MT0233 967
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 5 of 5
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, TetR family
No 50 0.12 0.809492 Antisense.TSS
Transcriptional regulator, TetR family
No -12 0.12 0.809492 CDS
Transcriptional regulator, TetR family
No -104 0.13 0.815286 Antisense.TSS
No 38 -0.19 0.735011 Antisense.TSS
Mycofactocin system transcriptional regulator
No 14 0.08 0.999588 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0122
e.value: 
1.1
Motif Bicluster: 
e.value: 
6.5
Motif Bicluster: 
0.57
bicluster_0147
e.value: 
160
Motif Bicluster: 
e.value: 
4800
Motif Bicluster: 
0.43
Product (LegacyBRC) Product (RefSeq)
PROBABLE ALDEHYDE DEHYDROGENASE aldehyde dehydrogenase
Operon # Operon
154
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Geraniol degradation

52
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607364 NP_214737.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.150000 0.87

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: