Rv0228 Lysophospholipid acyltransferase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0228 Lysophospholipid acyltransferase CDS 273055 274278 + 1 224 407 FALSE

Rv0228 (Lysophospholipid acyltransferase) is predicted to be co-regulated in modules bicluster_0242 with residual 0.54 and bicluster_0538 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0242 and 21.00 and 880.00 for bicluster_0538 respectively.

These modules are enriched for following go terms: DNA modification .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Re-Annotated Start Tuberculist Annotated Start
-0.636 273115 273055
Product (LegacyBRC) Product (RefSeq)
PROBABLE INTEGRAL MEMBRANE ACYLTRANSFERASE integral membrane acyltransferase
Operon # Operon
158
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Ethylbenzene degradation

11
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607369 NP_214742.1 Run
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: