Rv0242c 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0242c fabG4 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) CDS 290665 292029 - 1 365 454 FALSE

Rv0242c (3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)) is predicted to be co-regulated in modules bicluster_0011 with residual 0.41 and bicluster_0410 with residual 0.47.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.90 and 11.00 for bicluster_0011 and 1.20 and 4.10 for bicluster_0410 respectively.

These modules are enriched for following go terms: fatty acid synthase activity, transferase activity, transferring acyl ..., transferase activity, transferring acyl ... .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE 3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE FABG4 [3-KETOACYL-ACYL CARRIER PROTEIN REDUCTASE] 3-ketoacyl-(acyl-carrier-protein) reductase
Operon # Operon
168 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

3-oxoacyl-[acyl-carrier-protein] reductase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Fatty acid biosynthesis

13
Total items in this category:  

KEGG

Biosynthesis of unsaturated fatty acids

10
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607383 NP_214756.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
GO:0005618

cell wall

cell wall

Details: 
The rigid or semi-rigid envelope lying outside the cell membrane of plant, fungal, and most prokaryotic cells, maintaining their shape and protecting them from osmotic lysis. In plants it is made of cellulose and, often, lignin; in fungi it is composed largely of polysaccharides; in bacteria it is composed of peptidoglycan.
GO Category: 
cellular_component
320
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.390000 9.98

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: