Rv0309

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0309 CDS 377931 378587 + 657 218 FALSE

Rv0309 () is predicted to be co-regulated in modules bicluster_0157 with residual 0.53 and bicluster_0384 with residual 0.49.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0157 and 0.00 and 0.58 for bicluster_0384 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 18:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18724 MT0322 2903
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 4 of 4
ChipSeq TF Differential Expression Distance Expression pvalue Type
WhiB family transcriptional regulator
Induced -35 0.81 0.000679322 Primary.TSS
DNA-binding response regulator mtrA
No -43 0.31 0.627351 Primary.TSS
Transcriptional regulator, ArsR family
No -64 0.2 0.45151 Primary.TSS
Arsenical resistance operon repressor
Induced -77 0.65 0.0257658 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0157
e.value: 
0.000018
Motif Bicluster: 
e.value: 
0.000039
Motif Bicluster: 
0.53
bicluster_0384
e.value: 
0.0002
Motif Bicluster: 
e.value: 
0.58
Motif Bicluster: 
0.49
Product (LegacyBRC) Product (RefSeq)
POSSIBLE CONSERVED EXPORTED PROTEIN [Putative uncharacterized protein]
Operon # Operon
211
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607450 NP_214823.1 Run
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.700000 3.27

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: