Rv0318c Possible membrane protein

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0318c Possible membrane protein CDS 386305 387099 - 795 264 FALSE

Rv0318c (Possible membrane protein) is predicted to be co-regulated in modules bicluster_0438 with residual 0.51 and bicluster_0511 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.50 and 3,200.00 for bicluster_0438 and 670.00 and 6,900.00 for bicluster_0511 respectively.

These modules are enriched for following go terms: porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet..., cofactor biosynthetic process, sulfur amino acid biosynthetic process, sulfur amino acid metabolic process, cofactor metabolic process phosphopantetheine binding, modified amino acid binding, amide binding, amino acid binding.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 10 of 19
ChipSeq TF Differential Expression Distance Expression pvalue Type
Beta-carotene ketolase (EC 1.14.-.-)
No -21 0.34 0.999473 Internal.TSS
transcriptional regulator, ArsR family
No 47 0.29 0.106617 Antisense.TSS
Beta-carotene ketolase (EC 1.14.-.-)
No -31 0.34 0.999473 Antisense.TSS
Beta-carotene ketolase (EC 1.14.-.-)
No -47 0.34 0.999473 CDS
Transcriptional regulator, TetR family
Repressed -5 -1.03 0.0000687 CDS
Histone protein Lsr2
No 20 -0.06 0.926294 CDS
Two component transcriptional regulatory protein DevR
No 43 0.33 0.0450392 Internal.TSS
Transcriptional regulator, ArsR family
Repressed 17 -1.4 0.0000000000000233 Internal.TSS
transcriptional regulator, ArsR family
No 57 0.29 0.106617 Internal.TSS
Two component transcriptional regulatory protein DevR
No 33 0.33 0.0450392 Antisense.TSS
Motif 1 Motif 2 Residual
bicluster_0438
e.value: 
2.5
Motif Bicluster: 
e.value: 
3200
Motif Bicluster: 
0.51
bicluster_0511
e.value: 
670
Motif Bicluster: 
e.value: 
6900
Motif Bicluster: 
0.52
Product (LegacyBRC) Product (RefSeq)
PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN integral membrane protein
Operon # Operon
220 Rv0318c
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116721 YP_177716.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.430000 1.08

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.