Rv0321 Deoxycytidine triphosphate deaminase (EC 3.5.4.13)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0321 dcd Deoxycytidine triphosphate deaminase (EC 3.5.4.13) CDS 388582 389154 + 573 190 FALSE

Rv0321 (Deoxycytidine triphosphate deaminase (EC 3.5.4.13)) is predicted to be co-regulated in modules bicluster_0210 with residual 0.48 and bicluster_0491 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.02 and 29.00 for bicluster_0210 and 0.01 and 4,200.00 for bicluster_0491 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 18:54
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18388 MT0336 2164
Product (LegacyBRC) Product (RefSeq)
Deoxycytidine triphosphate deaminase deoxycytidine triphosphate deaminase
Operon # Operon
222 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

dCTP deaminase (dUMP-forming) Pyrimidine metabolism
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607462 NP_214835.1 Run
GO:0008829

dCTP deaminase activity

dCTP deaminase activity

Details: 
Catalysis of the reaction: dCTP + H2O = dUTP + NH3.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004170

dUTP diphosphatase activity

dUTP diphosphatase activity

Details: 
Catalysis of the reaction: dUTP + H2O = dUMP + diphosphate.
GO Category: 
molecular_function
2
Total items in this category:  
GO:0033973

dCTP deaminase (dUMP-forming) activity

dCTP deaminase (dUMP-forming) activity

Details: 
Catalysis of the reaction: dCTP + 2 H(2)O = diphosphate + dUMP + H(+) + NH(4)(+).
GO Category: 
molecular_function
1
Total items in this category:  
GO:0070207

protein homotrimerization

protein homotrimerization

Details: 
The formation of a protein homotrimer, a macromolecular structure consisting of three noncovalently associated identical subunits.
GO Category: 
biological_process
6
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.150000 0.12

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: