Rv0322 UDP-glucose dehydrogenase (EC 1.1.1.22)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0322 udgA UDP-glucose dehydrogenase (EC 1.1.1.22) CDS 389260 390591 + 1 332 443 FALSE

Rv0322 (UDP-glucose dehydrogenase (EC 1.1.1.22)) is predicted to be co-regulated in modules bicluster_0071 with residual 0.59 and bicluster_0113 with residual 0.50.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.04 and 0.76 for bicluster_0071 and 3.20 and 170.00 for bicluster_0113 respectively.

These modules are enriched for following go terms: exodeoxyribonuclease activity, exodeoxyribonuclease activity, producing..., exonuclease activity, active with either..., deoxyribonuclease activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
PROBABLE UDP-GLUCOSE 6-DEHYDROGENASE UDGA [UDP-GLC DEHYDROGENASE] [UDP-GLCDH] [UDPGDH] UDP-glucose 6-dehydrogenase UdgA
Operon # Operon
223
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Pentose and glucuronate interconversions

8
Total items in this category:  

KEGG

Ascorbate and aldarate metabolism

5
Total items in this category:  

KEGG

Starch and sucrose metabolism

20
Total items in this category:  

KEGG

Amino sugar and nucleotide sugar metabolism

28
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607463 NP_214836.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.330000 0.95

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: