Rv0337c Alanine transaminase (EC 2.6.1.2)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0337c aspC Alanine transaminase (EC 2.6.1.2) CDS 401873 403162 - 1 290 429 FALSE

Rv0337c (Alanine transaminase (EC 2.6.1.2)) is predicted to be co-regulated in modules bicluster_0020 with residual 0.58 and bicluster_0585 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 7.00 and 27,000.00 for bicluster_0020 and 0.06 and 350.00 for bicluster_0585 respectively.

These modules are enriched for following go terms: NADP binding cofactor biosynthetic process, coenzyme metabolic process, cofactor metabolic process.

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, MarR family
No -10 -0.26 0.133246 CDS
Mycofactocin system transcriptional regulator
No 6 0.06 0.999588 CDS
Transcriptional regulator, TetR family
No -56 0.05 0.927422 Internal.TSS
Motif 1 Motif 2 Residual
bicluster_0020
e.value: 
7
Motif Bicluster: 
e.value: 
27000
Motif Bicluster: 
0.58
bicluster_0585
e.value: 
0.055
Motif Bicluster: 
e.value: 
350
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Probable aspartate aminotransferase aminotransferase AlaT
Operon # Operon
233 Rv0337c - Rv0338c
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Valine, leucine and isoleucine biosynthesis

21
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607478 NP_214851.1 Run
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.