Rv0341 Isoniazid inductible protein IniB

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0341 iniB Isoniazid inductible protein IniB CDS 409362 410801 + 1 440 479 FALSE

Rv0341 (Isoniazid inductible protein IniB) is predicted to be co-regulated in modules bicluster_0409 with residual 0.46 and bicluster_0461 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.52 and 11.00 for bicluster_0409 and 23.00 and 620.00 for bicluster_0461 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Internal UTR New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.45 230 409592 -236 47 409309 409356 409362
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
transcriptional regulator, ArsR family
Induced 45 2.33 6.24e-25 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0409
e.value: 
0.52
Motif Bicluster: 
e.value: 
11
Motif Bicluster: 
0.46
bicluster_0461
e.value: 
23
Motif Bicluster: 
e.value: 
620
Motif Bicluster: 
0.55
Product (LegacyBRC) Product (RefSeq)
ISONIAZID INDUCTIBLE GENE PROTEIN INIB isoniazid inductible gene protein INIB
Operon # Operon
236 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607482 NP_214855.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.070000 0.55

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: