Rv0357c Adenylosuccinate synthetase (EC 6.3.4.4)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0357c purA Adenylosuccinate synthetase (EC 6.3.4.4) CDS 435471 436769 - 1 299 432 FALSE

Rv0357c (Adenylosuccinate synthetase (EC 6.3.4.4)) is predicted to be co-regulated in modules bicluster_0377 with residual 0.53 and bicluster_0569 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.17 for bicluster_0377 and 500.00 and 460.00 for bicluster_0569 respectively.

These modules are enriched for following go terms: purine-nucleoside phosphorylase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Adenylosuccinate synthetase adenylosuccinate synthetase
Operon # Operon
244 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Alanine, aspartate and glutamate metabolism

26
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607498 NP_214871.1 Run
GO:0004019

adenylosuccinate synthase activity

adenylosuccinate synthase activity

Details: 
Catalysis of the reaction: L-aspartate + GTP + IMP = N(6)-(1,2-dicarboxyethyl)-AMP + GDP + 3 H(+) + phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0040007

growth

growth

Details: 
The increase in size or mass of an entire organism, a part of an organism or a cell.
GO Category: 
biological_process
621
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: