Rv0389 Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0389 purT Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-) CDS 468335 469594 + 1 260 419 FALSE

Rv0389 (Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-)) is predicted to be co-regulated in modules bicluster_0035 with residual 0.52 and bicluster_0397 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 130.00 for bicluster_0035 and 0.00 and 0.00 for bicluster_0397 respectively.

These modules are enriched for following go terms: hydroxymethyl-, formyl- and related tran... nitrogen compound metabolic process, carbon-oxygen lyase activity.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.608 1256 469591 124 468295 468419 468335
Product (LegacyBRC) Product (RefSeq)
PROBABLE PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE 2 PURT [GART 2] [GAR TRANSFORMYLASE 2] [5&apos-PHOSPHORIBOSYLGLYCINAMIDE TRANSFORMYLASE 2] [FORMATE-DEPENDENT GAR TRANSFORMYLASE] phosphoribosylglycinamide formyltransferase 2
Operon # Operon
266 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

One carbon pool by folate

13
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607530 NP_214903.1 Run
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.030000 0.53

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: