Rv0405 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0405 pks6 Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39) CDS 485731 489939 + 4 209 1 402 FALSE

Rv0405 (Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)) is predicted to be co-regulated in modules bicluster_0303 with residual 0.47 and bicluster_0456 with residual 0.56.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 18,000.00 and 1,800.00 for bicluster_0303 and 190.00 and 9,400.00 for bicluster_0456 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18244 MT0418 1843
Product (LegacyBRC) Product (RefSeq)
PROBABLE MEMBRANE BOUND POLYKETIDE SYNTHASE PKS6 membrane bound polyketide synthase
Operon # Operon
275 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

[Acyl-carrier-protein] S-malonyltransferase Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607546 NP_214919.1 Run
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0044119

growth of symbiont in host cell

growth of symbiont in host cell

Details: 
The increase in size or mass of symbiont, occurring in its host's cell. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
50
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.470000 1.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: