Rv0409 Acetate kinase (EC 2.7.2.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0409 ackA Acetate kinase (EC 2.7.2.1) CDS 493851 495008 + 1 158 385 FALSE

Rv0409 (Acetate kinase (EC 2.7.2.1)) is predicted to be co-regulated in modules bicluster_0424 with residual 0.58 and bicluster_0565 with residual 0.53.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0424 and 0.16 and 35.00 for bicluster_0565 respectively.

These modules are enriched for following go terms: cellular macromolecule catabolic process, nucleobase-containing compound catabolic..., aromatic compound catabolic process, macromolecule catabolic process, exonuclease activity, active with either..., deoxyribonuclease activity, oxidoreductase activity, acting on paire..., exonuclease activity .

This gene is found to be non-essential for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator, ArsR family
No -87 -0.28 0.104139 CDS
Motif 1 Motif 2 Residual
bicluster_0424
e.value: 
1.4e-35
Motif Bicluster: 
e.value: 
7.6e-26
Motif Bicluster: 
0.58
bicluster_0565
e.value: 
0.16
Motif Bicluster: 
e.value: 
35
Motif Bicluster: 
0.53
Product (LegacyBRC) Product (RefSeq)
Acetate kinase acetate kinase
Operon # Operon
277 Rv0407 - Rv0408 - Rv0409
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Taurine and hypotaurine metabolism

7
Total items in this category:  

KEGG

Pyruvate metabolism

40
Total items in this category:  

KEGG

Propanoate metabolism

62
Total items in this category:  

KEGG

Methane metabolism

26
Total items in this category:  

KEGG

Carbon fixation pathways in prokaryotes

42
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607550 NP_214923.1 Run
GO:0008776

acetate kinase activity

acetate kinase activity

Details: 
Catalysis of the reaction: ATP + acetate = ADP + acetyl phosphate.
GO Category: 
molecular_function
1
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
Cholesterol Essentiality t-test p-value Cholesterol/Glycerol Ratio
non-essential 0.930000 2.40

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.