Rv0436c CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0436c pssA CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) CDS 524530 525390 - 861 286 FALSE

Rv0436c (CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8)) is predicted to be co-regulated in modules bicluster_0187 with residual 0.50 and bicluster_0407 with residual 0.55.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.01 and 0.16 for bicluster_0187 and 0.10 and 1.70 for bicluster_0407 respectively.

These modules are enriched for following go terms: organophosphate biosynthetic process, phospholipid metabolic process, phospholipid biosynthetic process GTPase activity, GTP binding, guanyl nucleotide binding, guanyl ribonucleotide binding.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
CDP-diacylglycerol--serine O-phosphatidyltransferase CDP-diacylglycerol--serine O-phosphatidyltransferase
Operon # Operon
292 - -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Glycine, serine and threonine metabolism

27
Total items in this category:  

KEGG

Glycerophospholipid metabolism

21
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607577 NP_214950.1 Run
GO:0003882

CDP-diacylglycerol-serine O-phosphatidyltransferase activity

CDP-diacylglycerol-serine O-phosphatidyltransferase activity

Details: 
Catalysis of the reaction: CDP-diacylglycerol + L-serine = CMP + O-sn-phosphatidyl-L-serine.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005576

extracellular region

extracellular region

Details: 
The space external to the outermost structure of a cell. For cells without external protective or external encapsulating structures this refers to space outside of the plasma membrane. This term covers the host cell environment outside an intracellular parasite.
GO Category: 
cellular_component
272
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.990000 2.47

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: