Rv0443

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0443 CDS 532396 532911 + 516 171 FALSE

Rv0443 () is predicted to be co-regulated in modules bicluster_0251 with residual 0.46 and bicluster_0389 with residual 0.58.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 780.00 and 7,200.00 for bicluster_0251 and 0.00 and 0.02 for bicluster_0389 respectively.

These modules are enriched for following go terms: cysteine metabolic process, L-serine metabolic process, serine family amino acid biosynthetic pr..., sulfur amino acid biosynthetic process, small molecule biosynthetic process, single-organism biosynthetic process, sulfur amino acid metabolic process, alpha-amino acid biosynthetic process mitochondrial electron transport, NADH t..., mitochondrial ATP synthesis coupled elec....

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18896 MT0459 3271
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No -12 -0.2 0.661641 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0251
e.value: 
780
Motif Bicluster: 
e.value: 
7200
Motif Bicluster: 
0.46
bicluster_0389
e.value: 
0.003
Motif Bicluster: 
e.value: 
0.017
Motif Bicluster: 
0.58
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
296
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607584 NP_214957.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.080000 1.08

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: