Rv0470c Mycolic acid synthase (PcaA/UmaA2) (Cyclopropane Synthase)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0470c pcaA Mycolic acid synthase (PcaA/UmaA2) (Cyclopropane Synthase) CDS 560848 561711 - 864 287 FALSE

Rv0470c (Mycolic acid synthase (PcaA/UmaA2) (Cyclopropane Synthase)) is predicted to be co-regulated in modules bicluster_0264 with residual 0.45 and bicluster_0390 with residual 0.52.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,300.00 and 940.00 for bicluster_0264 and 0.64 and 23.00 for bicluster_0390 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
MYCOLIC ACID SYNTHASE PCAA [CYCLOPROPANE SYNTHASE] mycolic acid synthase pcaA (cyclopropane synthase)
Operon # Operon
317
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
57116736 YP_177730.1 Run
GO:0008825

cyclopropane-fatty-acyl-phospholipid synthase activity

cyclopropane-fatty-acyl-phospholipid synthase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0042783

active evasion of host immune response

active evasion of host immune response

Details: 
Any mechanism of immune avoidance that directly affects the host immune system, e.g. blocking any stage in host MHC class I and II presentation. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
20
Total items in this category:  
GO:0046500

S-adenosylmethionine metabolic process

S-adenosylmethionine metabolic process

Details: 
The chemical reactions and pathways involving S-adenosylmethionine, S-(5'-adenosyl)-L-methionine, an important intermediate in one-carbon metabolism.
GO Category: 
biological_process
3
Total items in this category:  
GO:0052167

modulation by symbiont of host innate immune response

modulation by symbiont of host innate immune response

Details: 
Any process in which an organism modulates the frequency, rate or extent of the innate immune response of the host organism; the innate immune response is the host's first line of defense against infection. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
4
Total items in this category:  
GO:0071768

mycolic acid biosynthetic process

mycolic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycolic acids, beta-hydroxy fatty acids with a long alpha-alkyl side chain.
GO Category: 
biological_process
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.260000 0.45

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: