Rv0486 Glycosyltransferase MshA involved in mycothiol biosynthesis (EC 2.4.1.-)

Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0486 mshA Glycosyltransferase MshA involved in mycothiol biosynthesis (EC 2.4.1.-) CDS 575348 576790 + 1 443 480 FALSE

Rv0486 (Glycosyltransferase MshA involved in mycothiol biosynthesis (EC 2.4.1.-)) is predicted to be co-regulated in modules bicluster_0105 with residual 0.56 and bicluster_0482 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3,200.00 and 5,800.00 for bicluster_0105 and 0.02 and 0.93 for bicluster_0482 respectively.

These modules are enriched for following go terms: cellular macromolecule biosynthetic proc..., macromolecule biosynthetic process, macromolecule metabolic process, cytoplasm.

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.684 9 575357 454 574903 575357 575348
Product (LegacyBRC) Product (RefSeq)
Uncharacterized glycosyltransferase Rv0486_MT0504 mannosyltransferase
Operon # Operon
329 -
Locus Tuberculist Genome View


Locus Tag KEGG Pathways


not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network


GI Number Protein ID Blast Conserved Domains
15607627 NP_215000.1 Run

acetylglucosaminyltransferase activity

acetylglucosaminyltransferase activity

Catalysis of the transfer of an N-acetylglucosaminyl residue from UDP-N-acetyl-glucosamine to a sugar.
GO Category: 
Total items in this category:  

mycothiol biosynthetic process

mycothiol biosynthetic process

The chemical reactions and pathways resulting in the formation of mycothiol, which consists of N-acetyl-L-cysteine linked to a pseudodisaccharide, D-glucosamine and myo-inositol. Mycothiol is produced in actinomycetes like mycobacteria and serves similar functions to glutathione.
GO Category: 
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: