Rv0491 Phosphate regulon transcriptional regulatory protein PhoB (SphR); Sensory transduction protein regX3

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0491 regX3 Phosphate regulon transcriptional regulatory protein PhoB (SphR); Sensory transduction protein regX3 CDS 580809 581492 + 684 227 FALSE

Rv0491 (Phosphate regulon transcriptional regulatory protein PhoB (SphR); Sensory transduction protein regX3) is predicted to be co-regulated in modules bicluster_0061 with residual 0.63 and bicluster_0496 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0061 and 0.00 and 0.00 for bicluster_0496 respectively.

These modules are enriched for following go terms: isomerase activity sulfur compound metabolic process.

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 11/20/2020 - 12:11
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-14893 MT0510 182
Product (LegacyBRC) Product (RefSeq)
Sensory transduction protein regX3 two component sensory transduction protein RegX3
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Two-component system

53
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607632 NP_215005.1 Run
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0008150

biological_process

biological_process

Details: 
Any process specifically pertinent to the functioning of integrated living units: cells, tissues, organs, and organisms. A process is a collection of molecular events with a defined beginning and end.
GO Category: 
biological_process
66
Total items in this category:  
GO:0003677

DNA binding

DNA binding

Details: 
Any molecular function by which a gene product interacts selectively with DNA (deoxyribonucleic acid).
GO Category: 
molecular_function
37
Total items in this category:  
GO:0009405

pathogenesis

pathogenesis

Details: 
The set of specific processes that generate the ability of an organism to cause disease in another.
GO Category: 
biological_process
65
Total items in this category:  
GO:0006355

regulation of transcription, DNA-dependent

regulation of transcription, DNA-dependent

Details: 
Any process that modulates the frequency, rate or extent of cellular DNA-dependent transcription.
GO Category: 
biological_process
15
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.990000 1.85

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.015459
p-value INH: 0.988908
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 12.58 U
D3I 3 3 3.83 13.03 I
D3U 3 3 3.83 13.18 U
D5I 9 5 6.00 12.87 I
D5U 17 5 6.00 12.74 U
D7I 18 7 8.14 13.08 I
D7U 19 7 8.14 12.09 U
D14I 4 14 15.63 11.10 I
D14U 4 14 15.63 10.92 U
D17I 3 17 19.15 10.33 I
D17U 3 17 19.15 10.30 U
D21I 4 21 23.23 10.39 I
D21U 4 21 23.23 10.68 U
D24I 3 24 26.60 9.51 I
D24U 3 24 26.60 10.01 U
D28I 4 28 30.61 9.90 I
D28U 4 28 30.61 10.39 U