Rv0499 TesB-like acyl-CoA thioesterase 3

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0499 TesB-like acyl-CoA thioesterase 3 CDS 589183 590058 + 876 291 FALSE

Rv0499 (TesB-like acyl-CoA thioesterase 3) is predicted to be co-regulated in modules bicluster_0516 with residual 0.52 and bicluster_0591 with residual 0.62.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2,900.00 and 10,000.00 for bicluster_0516 and 56.00 and 610.00 for bicluster_0591 respectively.

These modules are enriched for following go terms: anion transport, external encapsulating structure, cell periphery, transmembrane transporter activity, transporter activity .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Uncharacterized protein Rv0499_MT0519
Operon # Operon
338 - - - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607640 NP_215013.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.060000 0.42

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: