Rv0503c Cyclopropane-fatty-acyl-phospholipid synthase 2, CmaA2 (EC 2.1.1.79)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0503c cmaA2 Cyclopropane-fatty-acyl-phospholipid synthase 2, CmaA2 (EC 2.1.1.79) CDS 593871 594779 - 909 302 FALSE

Rv0503c (Cyclopropane-fatty-acyl-phospholipid synthase 2, CmaA2 (EC 2.1.1.79)) is predicted to be co-regulated in modules bicluster_0164 with residual 0.60 and bicluster_0356 with residual 0.59.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 0.00 for bicluster_0164 and 0.00 and 0.00 for bicluster_0356 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-17890 MT0524 482
Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 3 of 3
ChipSeq TF Differential Expression Distance Expression pvalue Type
Transcriptional regulator kstR (Rv3574), TetR family
No -90 -0.11 0.717749 Primary.TSS
Transcriptional regulator, PadR family
No -114 0.16 0.744032 Primary.TSS
transcriptional regulator, ArsR family
Induced -97 0.71 0.00000513 Primary.TSS
Motif 1 Motif 2 Residual
bicluster_0164
e.value: 
5.2e-34
Motif Bicluster: 
e.value: 
3.9e-28
Motif Bicluster: 
0.60
bicluster_0356
e.value: 
1.8e-24
Motif Bicluster: 
e.value: 
1.4e-21
Motif Bicluster: 
0.59
Product (LegacyBRC) Product (RefSeq)
Cyclopropane-fatty-acyl-phospholipid synthase 2 cyclopropane-fatty-acyl-phospholipid synthase 2 CMAA2 (cyclopropane fatty acid synthase) (CFA synthase) (cyclopropane mycolic acid synthase 2) (mycolic acid trans-cyclopropane synthetase)
Operon # Operon
342 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607644 NP_215017.1 Run
GO:0001666

response to hypoxia

response to hypoxia

Details: 
Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating lowered oxygen tension. Hypoxia, defined as a decline in O2 levels below normoxic levels of 20.8 - 20.95%, results in metabolic adaptation at both the cellular and organismal level.
GO Category: 
biological_process
37
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0008825

cyclopropane-fatty-acyl-phospholipid synthase activity

cyclopropane-fatty-acyl-phospholipid synthase activity

Details: 
Catalysis of the reaction: S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.
GO Category: 
molecular_function
3
Total items in this category:  
GO:0046500

S-adenosylmethionine metabolic process

S-adenosylmethionine metabolic process

Details: 
The chemical reactions and pathways involving S-adenosylmethionine, S-(5'-adenosyl)-L-methionine, an important intermediate in one-carbon metabolism.
GO Category: 
biological_process
3
Total items in this category:  
GO:0052167

modulation by symbiont of host innate immune response

modulation by symbiont of host innate immune response

Details: 
Any process in which an organism modulates the frequency, rate or extent of the innate immune response of the host organism; the innate immune response is the host's first line of defense against infection. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
4
Total items in this category:  
GO:0071766

Actinobacterium-type cell wall biogenesis

Actinobacterium-type cell wall biogenesis

Details: 
A cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of a cell wall of the type found in Actinobacteria. The cell wall is the rigid or semi-rigid envelope lying outside the cell membrane. Actinobacterial cell walls contain characteristic mycolic acids, of which some are covalently linked to the cell wall peptidoglycan and others accumulate at the cell surface.
GO Category: 
biological_process
19
Total items in this category:  
GO:0071768

mycolic acid biosynthetic process

mycolic acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of mycolic acids, beta-hydroxy fatty acids with a long alpha-alkyl side chain.
GO Category: 
biological_process
15
Total items in this category:  
GO:0071769

mycolate cell wall layer assembly

mycolate cell wall layer assembly

Details: 
The aggregation, arrangement and bonding together of a set of components, including arabinogalactan mycolate and trehalose dimycolate, to form the mycolate layer of the Actinobacterium-type cell wall. The mycolate layer is physically attached to the peptidoglycan layer.
GO Category: 
biological_process
6
Total items in this category:  
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.050000 11.93

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: