Rv0517 Membrane acyltransferase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0517 Membrane acyltransferase CDS 608746 610056 + 1 311 436 FALSE

Rv0517 (Membrane acyltransferase) is predicted to be co-regulated in modules bicluster_0222 with residual 0.41 and bicluster_0596 with residual 0.61.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1.40 and 2.90 for bicluster_0222 and 0.00 and 0.03 for bicluster_0596 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

BASS Score Distance to Tuberculist Start Codon Internal TSS New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.979 1081 609827 108 608716 608824 608746
Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-18700 MT0538 2857
Product (LegacyBRC) Product (RefSeq)
POSSIBLE MEMBRANE ACYLTRANSFERASE membrane acyltransferase
Operon # Operon
349
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Benzoate degradation

48
Total items in this category:  

KEGG

Naphthalene degradation

40
Total items in this category:  

KEGG

Aminobenzoate degradation

73
Total items in this category:  

KEGG

Ethylbenzene degradation

11
Total items in this category:  

KEGG

Limonene and pinene degradation

65
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607658 NP_215031.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.810000 1.79

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: