Rv0520 Similar to methyltransferase domain of sarcosine-dimethylglycine methyltransferase

Summary
Product Feature Type Start End Strand Length AA Length is TF
Rv0520 Similar to methyltransferase domain of sarcosine-dimethylglycine methyltransferase CDS 612255 612605 + 351 116 FALSE

Rv0520 (Similar to methyltransferase domain of sarcosine-dimethylglycine methyltransferase) is predicted to be co-regulated in modules bicluster_0185 with residual 0.52 and bicluster_0449 with residual 0.57.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,500.00 and 29,000.00 for bicluster_0185 and 0.00 and 0.00 for bicluster_0449 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
POSSIBLE METHYLTRANSFERASE_METHYLASE
Operon # Operon
352 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Histidine metabolism

40
Total items in this category:  

KEGG

Tyrosine metabolism

41
Total items in this category:  

KEGG

Selenocompound metabolism

40
Total items in this category:  

KEGG

Polycyclic aromatic hydrocarbon degradation

47
Total items in this category:  

KEGG

Microbial metabolism in diverse environments

296
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607661 NP_215034.1 Run
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.410000 2.37

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: