Rv0533c \3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41)\\\""

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0533c fabH \3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41)\\\"" CDS 624473 625480 - 1 008 335 FALSE

Rv0533c (\3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.41)\\\"") is predicted to be co-regulated in modules bicluster_0123 with residual 0.55 and bicluster_0342 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 2.70 and 4,200.00 for bicluster_0123 and 37.00 and 4,800.00 for bicluster_0342 respectively.

These modules are enriched for following go terms: small molecule biosynthetic process, single-organism biosynthetic process, fatty acid biosynthetic process, carboxylic acid metabolic process, fatty acid synthase activity branched-chain amino acid biosynthetic p..., branched-chain amino acid metabolic proc..., cytosol, cytosolic part.

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
3-oxoacyl-[acyl-carrier-protein] synthase 3 3-oxoacyl-(acyl carrier protein) synthase III
Operon # Operon
359
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Beta-ketoacyl-acyl-carrier-protein synthase I Fatty acid biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607673 NP_215047.1 Run
GO:0000062

fatty-acyl-CoA binding

fatty-acyl-CoA binding

Details: 
Interacting selectively and non-covalently with acyl-CoA, any derivative of coenzyme A in which the sulfhydryl group is in thiolester linkage with a fatty acyl group.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0004315

3-oxoacyl-[acyl-carrier-protein] synthase activity

3-oxoacyl-[acyl-carrier-protein] synthase activity

Details: 
Catalysis of the reaction: acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein].
GO Category: 
molecular_function
3
Total items in this category:  
GO:0005515

protein binding

protein binding

Details: 
Interacting selectively and non-covalently with any protein or protein complex (a complex of two or more proteins that may include other nonprotein molecules).
GO Category: 
molecular_function
135
Total items in this category:  
GO:0006633

fatty acid biosynthetic process

fatty acid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of a fatty acid, any of the aliphatic monocarboxylic acids that can be liberated by hydrolysis from naturally occurring fats and oils. Fatty acids are predominantly straight-chain acids of 4 to 24 carbon atoms, which may be saturated or unsaturated; branched fatty acids and hydroxy fatty acids also occur, and very long chain acids of over 30 carbons are found in waxes.
GO Category: 
biological_process
12
Total items in this category:  
GO:0008610

lipid biosynthetic process

lipid biosynthetic process

Details: 
The chemical reactions and pathways resulting in the formation of lipids, compounds soluble in an organic solvent but not, or sparingly, in an aqueous solvent.
GO Category: 
biological_process
13
Total items in this category:  
GO:0030497

fatty acid elongation

fatty acid elongation

Details: 
The elongation of a fatty acid chain by the sequential addition of two-carbon units.
GO Category: 
biological_process
4
Total items in this category:  
GO:0033818

beta-ketoacyl-acyl-carrier-protein synthase III activity

beta-ketoacyl-acyl-carrier-protein synthase III activity

Details: 
Catalysis of the reaction: acetyl-CoA + malonyl-[acyl-carrier protein] = acetoacyl-[acyl-carrier protein] + CoA + CO2.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0035336

long-chain fatty-acyl-CoA metabolic process

long-chain fatty-acyl-CoA metabolic process

Details: 
The chemical reactions and pathways involving long-chain fatty-acyl-CoAs, any derivative of coenzyme A in which the sulfhydryl group is in a thioester linkage with a long-chain fatty-acyl group. Long-chain fatty-acyl-CoAs have chain lengths of C13 or more.
GO Category: 
biological_process
1
Total items in this category:  
GO:0042803

protein homodimerization activity

protein homodimerization activity

Details: 
Interacting selectively and non-covalently with an identical protein to form a homodimer.
GO Category: 
molecular_function
83
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.630000 1.71

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: