Rv0548c Naphthoate synthase (EC 4.1.3.36)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0548c menB Naphthoate synthase (EC 4.1.3.36) CDS 639012 639956 - 945 314 FALSE

Rv0548c (Naphthoate synthase (EC 4.1.3.36)) is predicted to be co-regulated in modules bicluster_0198 with residual 0.47 and bicluster_0210 with residual 0.48.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 13.00 and 3,100.00 for bicluster_0198 and 0.02 and 29.00 for bicluster_0210 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

BASS Score New Primary UTR Primary TSS Re-Annotated Start Tuberculist Annotated Start
-0.711 65 639979 639914 639956
Product (LegacyBRC) Product (RefSeq)
PROBABLE NAPHTHOATE SYNTHASE MENB [DIHYDROXYNAPHTHOIC ACID SYNTHETASE] [DHNA SYNTHETASE] naphthoate synthase
Operon # Operon
370
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Naphthoate synthase Ubiquinone and other terpenoid-quinone biosynthesis
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Ubiquinone and other terpenoid-quinone biosynthesis

7
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  

KEGG

Biosynthesis of secondary metabolites

326
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607688 NP_215062.1 Run
GO:0008935

1,4-dihydroxy-2-naphthoyl-CoA synthase activity

1,4-dihydroxy-2-naphthoyl-CoA synthase activity

Details: 
Catalysis of the reaction: O-succinylbenzoyl-CoA = 1,4-dihydroxy-2-naphthoate + CoA.
GO Category: 
molecular_function
1
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
GO:0034214

protein hexamerization

protein hexamerization

Details: 
The formation of a protein hexamer, a macromolecular structure consisting of six noncovalently associated identical or nonidentical subunits.
GO Category: 
biological_process
1
Total items in this category:  
GO:0051260

protein homooligomerization

protein homooligomerization

Details: 
The process of creating protein oligomers, compounds composed of a small number, usually between three and ten, of identical component monomers. Oligomers may be formed by the polymerization of a number of monomers or the depolymerization of a large protein polymer.
GO Category: 
biological_process
31
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: