Rv0550c Antitoxin 1a

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0550c vapB3 Antitoxin 1a CDS 640638 640904 - 267 88 FALSE

Rv0550c (Antitoxin 1a) is predicted to be co-regulated in modules bicluster_0051 with residual 0.43 and bicluster_0241 with residual 0.42.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.00 and 34.00 for bicluster_0051 and 5,500.00 and 8,700.00 for bicluster_0241 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?: Yes

Last update: 10/16/2017 - 02:57
BEI Mutant Available BEI Mutant ID BEI MT Number BEI Target ID Order from BEI
Yes NR-15028 MT0575 1196
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
371 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics

PATRIC

Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607690 NP_215064.1 Run
GO:0045926

negative regulation of growth

negative regulation of growth

Details: 
Any process that stops, prevents or reduces the rate or extent of growth, the increase in size or mass of all or part of an organism.
GO Category: 
biological_process
36
Total items in this category:  
GO:0075136

response to host

response to host

Details: 
Any process that results in a change in state or activity of the symbiont or its cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of detecting molecules of its host organism. The host is defined as the larger of the organisms involved in a symbiotic interaction.
GO Category: 
biological_process
12
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.550000 0.94

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: