Rv0555 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (EC 18.104.22.168)
|Symbol||Product||Feature Type||Start||End||Strand||Length||AA Length||is TF|
|Rv0555||menD||2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (EC 22.214.171.124)||CDS||646298||647962||+||1 665||554||FALSE|
Rv0555 (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (EC 126.96.36.199)) is predicted to be co-regulated in modules bicluster_0113 with residual 0.50 and bicluster_0438 with residual 0.51.
This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 3.20 and 170.00 for bicluster_0113 and 2.50 and 3,200.00 for bicluster_0438 respectively.
These modules are enriched for following go terms: exodeoxyribonuclease activity, exodeoxyribonuclease activity, producing..., exonuclease activity, active with either..., deoxyribonuclease activity porphyrin-containing compound metabolic ..., porphyrin-containing compound biosynthet..., cofactor biosynthetic process, sulfur amino acid biosynthetic process, sulfur amino acid metabolic process, cofactor metabolic process.
This gene is found to be non-essential for growth on cholesterol.
|ChipSeq TF||Differential Expression||Distance||Expression||pvalue||Type|
|Product (LegacyBRC)||Product (RefSeq)|
|2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase||2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase|
|BioCyc Gene Page||Cellular Overview Map|
|No results were found|
|Cholesterol Essentiality||t-test p-value||Cholesterol/Glycerol Ratio|
How essentiality calculations were done?
The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.