Rv0570 Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0570 nrdZ Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1) CDS 661295 663373 + 2 079 692 FALSE

Rv0570 (Ribonucleotide reductase of class II (coenzyme B12-dependent) (EC 1.17.4.1)) is predicted to be co-regulated in modules bicluster_0358 with residual 0.43 and bicluster_0404 with residual 0.54.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 720.00 and 2,100.00 for bicluster_0358 and 230.00 and 97.00 for bicluster_0404 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Ribonucleoside-diphosphate reductase ribonucleoside-diphosphate reductase large subunit NrdZ
Operon # Operon
384 -
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

Purine metabolism

65
Total items in this category:  

KEGG

Pyrimidine metabolism

41
Total items in this category:  

KEGG

Metabolic pathways

601
Total items in this category:  
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607710 NP_215084.1 Run
GO:0004748

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor

Details: 
Catalysis of the reaction: 2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin. Thioredoxin disulfide is the oxidized form of thioredoxin.
GO Category: 
molecular_function
4
Total items in this category:  
GO:0005829

cytosol

cytosol

Details: 
The part of the cytoplasm that does not contain organelles but which does contain other particulate matter, such as protein complexes.
GO Category: 
cellular_component
371
Total items in this category:  
GO:0005886

plasma membrane

plasma membrane

Details: 
The membrane surrounding a cell that separates the cell from its external environment. It consists of a phospholipid bilayer and associated proteins.
GO Category: 
cellular_component
1284
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.060000 0.91

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated:
p-value INH: