Rv0599c

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0599c vapB27 CDS 697564 697800 - 237 78 FALSE

Rv0599c () is predicted to be co-regulated in modules bicluster_0165 with residual 0.44 and bicluster_0179 with residual 0.46.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 0.03 and 0.07 for bicluster_0165 and 3,700.00 and 6,300.00 for bicluster_0179 respectively.

These modules are enriched for following go terms: phosphate-containing compound metabolic ..., phosphorus metabolic process, NAD binding .

This gene is found to be for growth on cholesterol.

Mutant available?:

Displaying 1 - 1 of 1
Gene Target Differential Expression Distance Expression pvalue Type
No results were found
Displaying 1 - 1 of 1
ChipSeq TF Differential Expression Distance Expression pvalue Type
No results were found
Motif 1 Motif 2 Residual
bicluster_0165
e.value: 
0.025
Motif Bicluster: 
e.value: 
0.071
Motif Bicluster: 
0.44
bicluster_0179
e.value: 
3700
Motif Bicluster: 
e.value: 
6300
Motif Bicluster: 
0.46
Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
400 -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607739 NP_215113.1 Run
Description:Expression data from transcription factor over expression experiments. TFOE are matched to the ChIP-seq experiment done simultaneously. This dataset is described in Rustad et al. 2014, Genome Biology.
BioProject Accession GEO Series References Repository Sample Method Sample Type
PRJNA254351 GSM1426512 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426513 GSE59086 25232098 GEO Tiling Array RNA
PRJNA254351 GSM1426514 GSE59086 25232098 GEO Tiling Array RNA
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
0.850000 1.00

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.00222263
p-value INH: 0.78494
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 12.59 U
D3I 3 3 3.83 12.86 I
D3U 3 3 3.83 12.96 U
D5I 9 5 6.00 11.63 I
D5U 17 5 6.00 11.38 U
D7I 18 7 8.14 12.70 I
D7U 19 7 8.14 12.06 U
D14I 4 14 15.63 13.31 I
D14U 4 14 15.63 11.95 U
D17I 3 17 19.15 12.95 I
D17U 3 17 19.15 11.39 U
D21I 4 21 23.23 12.98 I
D21U 4 21 23.23 11.37 U
D24I 3 24 26.60 12.35 I
D24U 3 24 26.60 10.71 U
D28I 4 28 30.61 12.58 I
D28U 4 28 30.61 10.44 U