Rv0608

Summary
Symbol Product Feature Type Start End Strand Length AA Length is TF
Rv0608 vapB28 CDS 703244 703489 + 246 81 FALSE

Rv0608 () is predicted to be co-regulated in modules bicluster_0060 with residual 0.34 and bicluster_0099 with residual 0.60.

This regulation is possibly mediated by two de-novo identified cis-regulatory motifs in each module with e-values , 1,700.00 and 1,700.00 for bicluster_0060 and 5.70 and 470.00 for bicluster_0099 respectively.

These modules are enriched for following go terms: .

This gene is found to be for growth on cholesterol.

Mutant available?:

Product (LegacyBRC) Product (RefSeq)
Putative uncharacterized protein
Operon # Operon
408 - -
PATRIC Locus Tag Enzyme Name PATRIC Pathways Transcriptomics
Not assigned Not assigned
Locus Tuberculist Genome View

Tuberculist

Quickview
Locus Tag KEGG Pathways

KEGG

not assigned to any KEGG Pathway.
BioCyc Gene Page Cellular Overview Map
Link to STRING STRING Network

STRING

GI Number Protein ID Blast Conserved Domains
15607748 NP_215122.1 Run
GO:0045927

positive regulation of growth

positive regulation of growth

Details: 
Any process that activates or increases the rate or extent of growth, the increase in size or mass of all or part of an organism.
GO Category: 
biological_process
31
Total items in this category:  
No TFOE experiment results were found
Quantitative Proteomics Data
t-test p-value Cholesterol/Glycerol Ratio
NA NA

How essentiality calculations were done?

The relative representation of each mutant was determined by calculating the fold change (sequence reads/insertion in cholesterol divided by sequence reads/insertion in glycerol) for each gene. Statistical significance was determined by t-test. Each insertion site in each replicate sample was treated as a separate data point. The hyperbola used for defining genes specifically required for growth in cholesterol was defined by the formula, y = 3.8/x+0.7. Genes above this line are annotated as required for growth on cholesterol.

TRIP log2 fold abundance change

reports the log2 abundance fold change of each TFI strain, relative to no induction, in absence or presence of drug, averaged across experimental replicates. Also reported are the accompanying z-scores and two-sided t-test p-values for each TFI strain under each condition. Please refer to Ma et al., 2020, Nature Microbiology for more information.

p-value Untreated: 0.0000696476
p-value INH: 0.725941
Displaying 1 - 17 of 17
Condition Count Day Doublings Fitness U.I Plots
D0U 27 0 0.00 11.18 U
D3I 3 3 3.83 11.28 I
D3U 3 3 3.83 10.77 U
D5I 9 5 6.00 10.76 I
D5U 17 5 6.00 10.34 U
D7I 18 7 8.14 11.89 I
D7U 19 7 8.14 11.12 U
D14I 4 14 15.63 11.63 I
D14U 4 14 15.63 10.50 U
D17I 3 17 19.15 11.58 I
D17U 3 17 19.15 10.07 U
D21I 4 21 23.23 11.78 I
D21U 4 21 23.23 10.22 U
D24I 3 24 26.60 11.65 I
D24U 3 24 26.60 9.72 U
D28I 4 28 30.61 11.75 I
D28U 4 28 30.61 9.77 U